Evaluation of in vitro activity of copper gluconate against SARS-CoV-2 using confocal microscopy-based high content screening
| Autor: | Killian Rodriguez, Sylvie Pillet, Yann Dickerscheit, Thomas Bourlet, Bruno Pozzetto, Josselin Rigaill, Amélie Prier, Florian Saunier, Elisabeth Botelho-Nevers, Estelle Audoux, Paul O. Verhoeven |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cell Survival
Green Fluorescent Proteins Context (language use) Biochemistry Antiviral Agents Gluconates Article Microbiology law.invention Green fluorescent protein Inorganic Chemistry chemistry.chemical_compound law Chlorocebus aethiops Animals Propidium iodide Viability assay Bovine serum albumin Vero Cells ComputingMethodologies_COMPUTERGRAPHICS Copper gluconate Microscopy Confocal biology SARS-CoV-2 Albumin COVID-19 In vitro chemistry High-content screening biology.protein Recombinant DNA Vero cell Vero E6 cells Molecular Medicine |
| Zdroj: | Journal of Trace Elements in Medicine and Biology |
| ISSN: | 1878-3252 |
| Popis: | Graphical abstract Context Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) that emerged late in 2019 is the etiologic agent of coronavirus disease 2019 (Covid-19). There is an urgent need to develop curative and preventive therapeutics to limit the current pandemic and to prevent the re-emergence of Covid-19. This study aimed to assess the in vitro activity of copper gluconate against SARS-CoV-2. Methods Vero E6 cells were cultured with or without copper gluconate 18−24 hours before infection. Cells were infected with a recombinant GFP expressing SARS-CoV-2. Cells were infected with a recombinant GFP expressing SARS-CoV-2. Infected cells were incubated in fresh medium containing varying concentration of copper gluconate (supplemented with bovine serum albumin or not) for an additional 48 -h period. The infection level was measured by the confocal microscopy-based high content screening method. The cell viability in presence of copper gluconate was assessed by XTT and propidium iodide assays. Results The viability of Vero E6 cells exposed to copper gluconate up to 200 μM was found to be similar to that of unexposed cells, but it dropped below 70 % with 400 μM of this agent after 72 h of continuous exposure. The infection rate was 23.8 %, 18.9 %, 20.6 %, 6.9 %, 5.3 % and 5.2 % in cells treated prior infection with 0, 2, 10, 25, 50 and 100 μM of copper gluconate respectively. As compared to untreated cells, the number of infected cells was reduced by 71 %, 77 %, and 78 % with 25, 50, and 100 μM of copper gluconate respectively (p < 0.05). In cells treated only post-infection, the rate of infection dropped by 73 % with 100 μM of copper gluconate (p < 0.05). However, the antiviral activity of copper gluconate was abolished by the addition of bovine serum albumin. Conclusion Copper gluconate was found to mitigate SARS-CoV-2 infection in Vero E6 cells but this effect was abolished by albumin, which suggests that copper will not retain its activity in serum. Furthers studies are needed to investigate whether copper gluconate could be of benefit in mucosal administration such as mouthwash, nasal spray or aerosols. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect