Non-disulfide-Bridge Peptide 5.5 from the Scorpion Hadrurus gertschi Inhibits the Growth of Mycobacterium abscessus subsp. massiliense
| Autor: | Monalisa Martins Trentini, Roosevelt A. DaSilva, Márcia Renata Mortari, André Kipnis, Adolfo Carlos Barros de Souza, Bruno de Paula Oliveira Santos, Ana Paula Junqueira-Kipnis, Elisabeth F. Schwartz, Rogério Coutinho das Neves |
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| Rok vydání: | 2017 |
| Předmět: |
Microorganismos
0301 basic medicine Microbiology (medical) medicine.drug_class 030106 microbiology Antimicrobial peptides Mycobacterium abscessus Antimycobacterial Microbiology 03 medical and health sciences medicine Original Research AMP Escorpião - veneno Minimum bactericidal concentration Peptídeos biology non-disulfide bridged peptide biology.organism_classification Antimicrobial medicine.disease Virology infection Hemolysis rapid-growing mycobacteria 030104 developmental biology scorpion venom antimycobacterial agents Agentes antibacterianos Bacteria Infecção Mycobacterium |
| Zdroj: | Repositório Institucional da UnB Universidade de Brasília (UnB) instacron:UNB Frontiers in Microbiology |
| ISSN: | 1664-302X |
| DOI: | 10.3389/fmicb.2017.00273 |
| Popis: | Multi-drug resistant microorganisms have been a growing concern during the last decades due to their contribution in mortality rates worldwide. Antimicrobial peptides are broad spectrum antimicrobial agents that display potent microbicidal activity against a wide range of microorganisms. Antimicrobial peptides generally have a rapid mode of action that reduces the risk of resistance developing among pathogens. In this study, an antimicrobial peptide derived from scorpion venom, NDBP-5.5, was evaluated against Mycobacterium abscessus subsp. massiliense, a rapidly growing and emerging pathogen associated with healthcare infections. The minimal bactericidal concentration of NDBP-5.5, AMP quantity necessary to stop bacteria visible growth, against M. abscessus subsp. massiliense was 200 μM, a concentration that did not induce hemolysis of human red blood cells. The therapeutic index was 3.05 indicating a drug with low toxicity and therefore good clinical potential. Treatment of infected macrophages with NDBP-5.5 or clarithromycin presented similar results, reducing the bacterial load. M. abscessus subsp. massiliense-infected animals showed a decrease in the bacterial load of up to 70% when treated with NDBP-5.5. These results revealed the effective microbicidal activity of NDBP-5.5 against mycobacterium, indicating its potential as an antimycobacterial agent. |
| Databáze: | OpenAIRE |
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