Expression of Ca2+-dependent Synaptotagmin Isoforms in Mouse and Rat Parotid Acinar Cells
| Autor: | Min Seuk Kim, Seung Hyeoi Kim, Jeong Taeg Seo, Syng Ill Lee, Dong Min Shin, Heung Kyu Son, Hae Mi Byun, Hae Jo, Jeong Hee Hong, Jong Hoon Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_specialty
Stimulation Muscarinic Agonists calcium signaling Synaptic vesicle Exocytosis Synaptotagmin 1 Synaptotagmins Mice stomatognathic system Internal medicine Muscarinic acetylcholine receptor medicine Animals Parotid Gland Protein Isoforms parotid acinar cells Chemistry General Medicine Synaptotagmin Parotid gland Cell biology Rats stomatognathic diseases medicine.anatomical_structure Endocrinology Amylases Original Article Calcium Carbachol Signal transduction Signal Transduction |
| Zdroj: | Yonsei Medical Journal |
| ISSN: | 1976-2437 0513-5796 |
| Popis: | Synaptotagmin is a Ca2+ sensing protein, which triggers a fusion of synaptic vesicles in neuronal transmission. Little is known regarding the expression of Ca2+-dependent synaptotagmin isoforms and their contribution to the release of secretory vesicles in mouse and rat parotid acinar cells. We investigated a type of Ca2+-dependent synaptotagmin and Ca2+ signaling in both rat and mouse parotid acinar cells using RT-PCR, microfluorometry, and amylase assay. Mouse parotid acinar cells exhibited much more sensitive amylase release in response to muscarinic stimulation than did rat parotid acinar cells. However, transient [Ca2+]i increases and Ca2+ influx in response to muscarinic stimulation in both cells were identical, suggesting that the expression or activity of the Ca2+ sensing proteins is different. Seven Ca2+-dependent synaptotagmins, from 1 to 7, were expressed in the mouse parotid acinar cells. However, in the rat parotid acinar cells, only synaptotagmins 1, 3, 4 and 7 were expressed. These results indicate that the expression of Ca2+-dependent synaptotagmins may contribute to the release of secretory vesicles in parotid acinar cells. |
| Databáze: | OpenAIRE |
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