Regulation of lipoxygenase-1 and Dectin-1 on interleukin-10 in mouse Aspergillus fumigatus keratitis
| Autor: | Kelan Yuan, Min Liu, Chengye Che, Guoqiang Zhu, Cui Li, Guiqiu Zhao, Jing Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
interleukin-10 Microbiology law.invention Aspergillus fumigatus Keratitis 03 medical and health sciences Lipoxygenase Western blot lcsh:Ophthalmology law Medicine skin and connective tissue diseases Polymerase chain reaction Messenger RNA biology medicine.diagnostic_test business.industry Sterile water biology.organism_classification medicine.disease Ophthalmology Interleukin 10 030104 developmental biology Basic Research keratitis lcsh:RE1-994 lipoxygenase-1 biology.protein business Dectin-1 |
| Zdroj: | International Journal of Ophthalmology, Vol 11, Iss 6, Pp 905-909 (2018) |
| ISSN: | 2227-4898 2222-3959 |
| Popis: | AIM: To investigate the regulation of lipoxygenase (LOX)-1 and Dectin-1 on interleukin-10 (IL-10) production in mice with Aspergillus fumigatus (A. fumigatus) keratitis. METHODS: The corneas of C57BL/6 mice were pretreated with LOX-1 inhibitor Poly(I) or Dectin-1 siRNA separately before the infection of A. fumigatus. Polymerase chain reaction (PCR) and Western blot were used to detect the expression of IL-10. RESULTS: The mRNA and protein expressions of IL-10 were significantly increased in mice with A. fumigatus keratitis. Compared with the group pretreated with sterile water before infection, Poly(I) pretreatment suppressed IL-10 expression significantly. Compared with the group pretreated with scrambled siRNA before infection, Dectin-1 siRNA pretreatment significantly reduced IL-10 expression in response to A. fumigatus infection. CONCLUSION: LOX-1 and Dectin-1 regulate IL-10 production in mouse A. fumigatus keratitis. |
| Databáze: | OpenAIRE |
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