Anti-inflammatory effects of a triterpenoid isolated from Wilbrandia ebracteata Cogn
| Autor: | Rosa Maria Ribeiro-do-Valle, Andressa Córneo Gazola, Giles A. Rae, Patricia Baier Krepsky, Rodrigo Rebelo Peters, Artur J. de Brum-Fernandes, Mareni Rocha Farias, Jarbas Mota Siqueira |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Neutrophils medicine.drug_class Stimulation Inflammation Pharmacology Carrageenan Leukotriene B4 Plant Roots Dinoprostone General Biochemistry Genetics and Molecular Biology Anti-inflammatory Mice chemistry.chemical_compound Cucurbitacins In vivo Chlorocebus aethiops Animals Edema Humans Medicine Cyclooxygenase Inhibitors General Pharmacology Toxicology and Pharmaceutics Pleurisy Methylene Chloride Cyclooxygenase 2 Inhibitors business.industry Anti-Inflammatory Agents Non-Steroidal General Medicine Transfection Triterpenes In vitro Cucurbitaceae chemistry COS Cells Immunology Cyclooxygenase 1 NIH 3T3 Cells Solvents medicine.symptom business |
| Zdroj: | Life Sciences. 80:1382-1387 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2006.12.021 |
| Popis: | Wilbrandia ebracteata (WE), a Brazilian medicinal plant used in folk medicine for the treatment of rheumatic diseases, displays anti-inflammatory properties and constitutes a rich source of cucurbitacins and cucurbitacin-related compounds. The current study investigated the potential anti-inflammatory properties of Dihydrocucurbitacin B (DHCB), a cucurbitacin-derived compound isolated from roots of WE, in some in vivo and in vitro experimental models. Intraperitoneal treatment of mice with DHCB reduced both carrageenan-induced paw edema (0.3, 1 and 3 mg/kg caused inhibitions of 26, 44 and 56 % at 2 h after stimulation, respectively) and pleurisy (10 mg/kg inhibited leukocyte numbers and LTB(4) levels in the pleural fluid by 51 and 75% at 6 h after cavity challenge, respectively). In vitro, DHCB (up to 10 microg/mL) failed to modify LTB(4) production by human neutrophils or PGE(2) production by COS-7 cells transfected with COX-1, but PGE(2) production by COX-2 transfected COS-7 cells was markedly inhibited (by 72%). The levels of COX-1 or COX-2 proteins in IL-1alpha-stimulated NIH3T3 cells were unaffected by DHCB. The results corroborate the potential anti-inflammatory properties ascribed to W. ebracteata Cogn. in folk medicine and suggest that they might be attributed, at least in part, to the capacity of one of this plants main constituents, DHCB, to inhibit COX-2 activity (but not its expression) during inflammation. |
| Databáze: | OpenAIRE |
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