Embryo catheter loading and embryo culture techniques: results of a worldwide web-based survey
| Autor: | Zeev Shoham, Milton Leong, Mindy S. Christianson, Anat Safran, Ayatallah M. Khafagy, Gon Shoham, Irit Granot, Yulian Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
animal structures
medicine.medical_treatment Fertilization in Vitro Biology Bioinformatics Catheterization Andrology Embryo Culture Techniques Embryo Culture Technique Genetics medicine Humans Embryo Implantation Assisted Reproduction Technologies Genetics (clinical) Retrospective Studies Internet In vitro fertilisation Obstetrics and Gynecology International Agencies Embryo culture Embryo General Medicine Embryo Transfer Embryo transfer Catheter Blastocyst Reproductive Medicine embryonic structures Female Developmental Biology |
| Popis: | To identify trends in embryo catheter loading and embryo culture techniques performed worldwide.A retrospective evaluation using the results of a web-based survey, (IVF Worldwide ( www.IVF-worldwide.com ), was performed.Responses from 265 centers in 71 countries were obtained. Most centers (97 %) preferred a catheter with its orifice on top, with only 3 % preferring a catheter with the orifice on its side; 41 % preferred a catheter marked for clear ultrasound view. The most commonly-reported methods of embryo loading were medium-air-embryo-air-medium (42 %), medium in catheter with embryo at end (20 %) and medium-air-embryo (15 %). In 68 % of centers the final volume of the catheter was up to 0.3 ml, with only 19 % using 0.3-0.5 ml and 1 % using 0.5-0.7 ml. Using reduced oxygen concentrations for embryo culture was divided between those who used it in combination with the two-gas system (34 %) and those who did not use it at all (39 %); 24 % reported using a three-gas system. Most clinics using reduced oxygen concentrations used it throughout the entire culture period. Half of centers (51 %) reported using reduced oxygen concentrations for the entire IVF population while 6 % reserved it only for blastocyst transfer. The use of sequential media was highly dominant with 40 % reporting its use. |
| Databáze: | OpenAIRE |
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