Mannan oligosaccharides supplementation enhanced head-kidney and spleen immune function in on-growing grass carp (Ctenopharyngodon idella)
| Autor: | Pei Wu, Juan Yang, Lin Feng, Ling Tang, Wei-Dan Jiang, Yang Liu, Sheng-Yao Kuang, Xiao-Qiu Zhou, Zhi-Yuan Lu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Carps Antimicrobial peptides Oligosaccharides Spleen Aquatic Science Mannans Fish Diseases Random Allocation 03 medical and health sciences Immune system Internal medicine medicine Animals Environmental Chemistry Mannan Dose-Response Relationship Drug biology Interleukin 04 agricultural and veterinary sciences General Medicine Head Kidney biology.organism_classification Animal Feed Aeromonas hydrophila Diet Grass carp 030104 developmental biology Endocrinology medicine.anatomical_structure Dietary Supplements 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Antibody Gram-Negative Bacterial Infections Signal Transduction |
| Zdroj: | Fish & Shellfish Immunology. 106:596-608 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2020.08.035 |
| Popis: | This research investigates the influences of dietary mannan oligosaccharides (MOS) on the head-kidney and spleen immune function in on-growing grass carp (Ctenopharyngodon idella) and its related mechanism. Fish were fed during 60 days at different levels of MOS (0, 200, 400, 600, 800 and 1000 mg kg−1). Subsequently, 14 days after the feeding trial was injected intraperitoneally with Aeromonas hydrophila, the immune function was studied. The results are as follows: (1) appropriate MOS supplementation could increase the content of antibacterial compound and immunoglobulin (Ig), up-regulate antimicrobial peptides transcriptional levels in these two organs; (2) appropriate MOS supplementation attenuated inflammatory response in these two organs by regulating cytokines (pro- and anti-inflammatory cytokines) and related signalling pathways (NF-κB and TOR). The interesting points though, was, no differences were found in liver-expressed antimicrobial peptide (LEAP)-2A, interleukin (IL)-8, IL-4/13B, IκB kinase (IKK) α and nuclear factor kappa B (NF-κB) p52 gene expression in these two organs. Consequently, the present research suggests that MOS supplementation can enhance head-kidney and spleen immune function. Finally, we obtained these appropriate MOS dose (538.5 and 585.8 mg kg−1) by quadratic regression analysis of lysozyme activity (head-kidney) and phosphatase activity (spleen), respectively. |
| Databáze: | OpenAIRE |
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