Development of an Integrated Syringe-Pump-Based Environmental-Water Analyzer (iSEA) and Application of It for Fully Automated Real-Time Determination of Ammonium in Fresh Water
| Autor: | Jian Ma, Peicong Li, Nengwang Chen, Chen Zhaoying, Jixin Chen, Kunning Lin, Dongxing Yuan, Yiyong Jiang, Bangqin Huang |
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| Rok vydání: | 2018 |
| Předmět: |
Spectrum analyzer
Calibration curve Analytical chemistry Fresh Water 02 engineering and technology 01 natural sciences Analytical Chemistry chemistry.chemical_compound Rivers Limit of Detection Ammonium Compounds Ammonium Flow injection analysis Syringe driver Detection limit Chemistry Syringes Biphenyl Compounds 010401 analytical chemistry Equipment Design 021001 nanoscience & nanotechnology 0104 chemical sciences Biphenyl compound Indophenol Reagent Flow Injection Analysis 0210 nano-technology Environmental Monitoring |
| Zdroj: | Analytical Chemistry. 90:6431-6435 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.8b01490 |
| Popis: | The development of a multipurpose integrated syringe-pump-based environmental-water analyzer ( iSEA) and its application for spectrophotometric determination of ammonium is presented. The iSEA consists of a mini-syringe pump equipped with a selection valve and laboratory-programmed software written by LabVIEW. The chemistry is based on a modified indophenol method using o-phenylphenol. The effect of reagent concentrations and sample temperatures was evaluated. This fully automated analyzer had a detection limit of 0.12 μM with sample throughput of 12 h-1. Relative standard deviations at different concentrations (0-20 μM) were 0.23-3.36% ( n = 3-11) and 1.0% ( n = 144, in 24 h of continuous measurement, ∼5 μM). Calibration curves were linear ( R2 = 0.9998) over the range of 0-20 and 0-70 μM for the detection at 700 and 600 nm, respectively. The iSEA was applied in continuous real-time monitoring of ammonium variations in a river for 24 h and 14 days. A total of 1802 samples were measured, and only 0.4% was outlier data (≥3 sigma residuals). Measurements of reference materials and different aqueous samples ( n = 26) showed no significant difference between results obtained by reference and present methods. The system is compact (18 cm × 22 cm × 24 cm), portable (4.8 kg), and robust (high-resolution real-time monitoring in harsh environments) and consumes a small amount of chemicals (20-30 μL/run) and sample/standards (2.9 mL/run). |
| Databáze: | OpenAIRE |
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