Application of IGF2 – Specific Identifier Probe for Cytogenetic Study of Somatic and Sperm Cells in Horses
| Autor: | Marek Tischner, Leszek Potocki, Monika Bugno-Poniewierska, Maciej Wnuk |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.diagnostic_test Somatic cell Locus (genetics) General Medicine Biology Spermatozoa Sperm Molecular biology General Biochemistry Genetics and Molecular Biology Chromatin Insulin-Like Growth Factor II medicine Animals Interphase Horses Metaphase Gene In Situ Hybridization Fluorescence Fluorescence in situ hybridization |
| Zdroj: | Folia Biologica. 59:147-149 |
| ISSN: | 0015-5497 |
| DOI: | 10.3409/fb59_3-4.147-149 |
| Popis: | The development of molecular techniques with fluorochromes has had an invaluable impact on discovering the nature of chromatin structure. Here, we show the application of a locus specific identifier probe (LSI) for precise and selective visualization of the horse IGF2 gene in the metaphase, interphase nuclei and sperm cells. Our study may be helpful for interpretation of results of interphase fluorescence in situ hybridization (I-FISH). We analyze and discuss the variation in the number and localization of FISH signals in somatic and sperm cells of horse. |
| Databáze: | OpenAIRE |
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