Circular RNA Paired-Related Homeobox 1 Promotes Gastric Carcinoma Cell Progression via Regulating MicroRNA-665/YWHAZ Axis
| Autor: | Weigao Hu, Wei Liu, Song Zhu, Kezhu Hou |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Physiology Cell Mice Nude Apoptosis Flow cytometry Mice Downregulation and upregulation Cell Movement Stomach Neoplasms Cell Line Tumor microRNA medicine Animals Humans Cell Proliferation Homeodomain Proteins Messenger RNA Gene knockdown medicine.diagnostic_test Chemistry Carcinoma Gastroenterology Neoplasms Experimental Molecular biology Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs medicine.anatomical_structure 14-3-3 Proteins Gene Knockdown Techniques YWHAZ |
| Zdroj: | Digestive Diseases and Sciences. 66:3842-3853 |
| ISSN: | 1573-2568 0163-2116 |
| DOI: | 10.1007/s10620-020-06705-5 |
| Popis: | Gastric carcinoma (GC) is a ubiquitous malignant tumor worldwide. Circular RNA paired-related homeobox 1 (circ-PRRX1), one kind of non-coding RNAs, has been reported to act as a promoter in tumor growth. This study aims to explore the effects of circ-PRRX1 on proliferation, apoptosis, and metastasis in GC and the underlying regulatory mechanisms. The expression of circ-PRRX1, miR-665, and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta (YWHAZ) mRNA was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Western blot was used to analyze YWHAZ protein expression. 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-Htetrazolium bromide (MTT), flow cytometry, and transwell assay were carried out to assess the viability, apoptosis, migration, and invasion in GC cells. The interaction between miR-665 and circ-PRRX1 or YWHAZ was predicted by StarBase v2.0 and identified by dual-luciferase reporter system. Xenograft mouse model was employed to determine the effects of circ-PRRX1 knockdown on GC growth in vivo. Compared with normal tissues and cells, circ-PRRX1 and YWHAZ levels were upregulated, and miR-665 was downregulated in GC tissues and cells. Functionally, circ-PRRX1 knockdown inhibited the viability, migration, and invasion and promoted apoptosis in GC cells, whereas anti-miR-665 abolished these effects. Mechanistically, circ-PRRX1 was confirmed as a sponge of miR-665 to regulate YWHAZ expression. Xenograft mouse model suggested that circ-PRRX1 knockdown reduced GC cells growth in vivo. Circ-PRRX1 knockdown suppressed GC development by targeting miR-665 to inhibit YWHAZ expression, and the potential molecular mechanism may provide a theoretical basis for GC therapy. |
| Databáze: | OpenAIRE |
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