Protein Hydrolysate of Silkworm Pupa Prevents Memory Impairment Induced by Oxidative Stress in Scopolamine-Induced Mice via Modulating the Cholinergic Nervous System and Antioxidant Defense System
| Autor: | Jong-Hoon Kim, Seung Yeon Baek, Fu Yi Li, Hun Jung Kim, Chang-Won Ahn, Mee Ree Kim |
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| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
medicine.medical_treatment Morris water navigation task Oxidative phosphorylation Pharmacology cholinergic nervous system medicine.disease_cause Neuroprotection Article 03 medical and health sciences silkworm pupae 0302 clinical medicine antioxidant enzymes medicine Viability assay 030304 developmental biology 0303 health sciences Nutrition and Dietetics Chemistry fungi Glutamate receptor memory enhancing effect 030220 oncology & carcinogenesis Cholinergic Oxidative stress Food Science |
| Zdroj: | Preventive Nutrition and Food Science |
| ISSN: | 2287-8602 2287-1098 |
| Popis: | Silkworm pupae (Bombyx mori) is an edible insect that has been reported to contain high-quality proteins, lipids, minerals, and vitamins, and to possess high antioxidant activity. However, there have been no studies on the neuroprotective effects of silkworm pupae. Therefore, we investigated a water extract of silkworm pupae with protease (WSP) as a functional and therapeutic candidate for neurodegenerative disorders. First, we evaluated the effect of WSP on oxidative stress-induced mouse hippocampal neuronal cells (HT-22 cells). Cell viability diminished by addition of glutamate but was significantly recovered by WSP treatment. Furthermore, WSP significantly decreased the release of lactate dehydrogenase and generation of intracellular reactive oxygen species in oxidative stress-induced cells. In addition, in scopolamine-treated mice, WSP attenuated memory impairment, as demonstrated in the Morris water maze and passive avoidance tests, indicating protection of neuronal cells against oxidative damage. Moreover, WSP prevented scopolamine-induced increases in acetylcholinesterase activity and decreases in choline-acetyltransferase activity. Finally, treatment with WSP enhanced the antioxidant defense system by regulating the activities of antioxidant enzymes. Overall, this study showed that WSP exerted antioxidant and memory enhancing action against oxidative stress. |
| Databáze: | OpenAIRE |
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