Quantitative evaluation of auraptene and umbelliferone, chemopreventive coumarins in citrus fruits, by HPLC-UV-FL-MS
| Autor: | Vittorio Sorella, Anna Ferranti, Roberto Mandrioli, Francesco Epifano, Michele Protti, Laura Mercolini, Maria Augusta Raggi, Massimo Curini |
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| Přispěvatelé: | Laura Mercolini, Roberto Mandrioli, Anna Ferranti, Vittorio Sorella, Michele Protti, Francesco Epifano, Massimo Curini, Maria Augusta Raggi |
| Rok vydání: | 2012 |
| Předmět: |
Citrus
Chromatography Chemistry Plant Extracts citrus fruit General Chemistry Umbelliferone HPLC-UV-FL Mass spectrometric High-performance liquid chromatography Mass Spectrometry umbelliferone chemistry.chemical_compound Nutraceutical quali-quantitative analysi auraptene Coumarins Fruit Auraptene Analytical strategy nutraceutical Umbelliferones General Agricultural and Biological Sciences Quantitative analysis (chemistry) Chromatography High Pressure Liquid |
| Zdroj: | Journal of agricultural and food chemistry. 61(8) |
| ISSN: | 1520-5118 |
| Popis: | An analytical strategy, based on the development of two HPLC methods with spectrophotometric (UV), spectrofluorometric (FL), and mass spectrometric (MS) detection, has been developed to investigate the presence of and to quantitate two important chemopreventive coumarins, auraptene and umbelliferone, in foodstuffs. The analytes were determined in fruits, and fruit parts, of plants belonging to the Citrus , Poncirus , and Fortunella genera, to test their nutraceutical potential. The method validation has been carried out according to international guidelines, with good results in terms of precision (RSD6.9%) and extraction yields (91%). Application to the quantitative analysis of auraptene and umbelliferone in several kinds of citrus fruits was successful, providing reliable and consistent data. Exploiting three different kinds of detection, the analytical methodology proposed herein has been demonstrated to be sound but versatile, as well as reliable. Performances and results were compared and always found in good agreement among themselves. Thus, this approach is suitable for the identification and simultaneous quantitation of auraptene and umbelliferone in citrus fruits, with the aim of evaluating their nutraceutical potential. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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