Constitutive Endothelial Nitric Oxide Synthase (ecNOS) Gene Expression in Human Monocytes
| Autor: | A. Antonello, Umberto Fagiolo, Monia Milani, Lorenzo Calo, Paul A. Davis, Salvatore Cantaro, Angela D'Angelo |
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| Rok vydání: | 1998 |
| Předmět: |
Gene isoform
Nitric Oxide Synthase Type III Endothelium Sequence analysis Cell Gene Expression 030204 cardiovascular system & hematology Polymerase Chain Reaction Monocytes law.invention 03 medical and health sciences 0302 clinical medicine law Gene expression medicine Humans RNA Messenger 030212 general & internal medicine Polymerase chain reaction biology business.industry Monocyte Molecular biology Isoenzymes Nitric oxide synthase medicine.anatomical_structure biology.protein Nitric Oxide Synthase Cardiology and Cardiovascular Medicine business |
| Zdroj: | Angiology. 49:419-422 |
| ISSN: | 1940-1574 0003-3197 |
| DOI: | 10.1177/000331979804900601 |
| Popis: | This study evaluates using a polymerase chain reaction (PCR)-based molecular biological approach to study the gene expression of the constitutive endothelial isoform of nitric oxide synthase (ecNOS) in human monocytes. When PCR was carried out with specific primers for ecNOS, 302 bp product was amplified, which sequence analysis determined as having 100% identity to the reported human ecNOS isoform gene sequence. The data presented here demonstrate a reliable technique for assessing ecNOS mRNA levels in circulating human monocytes. This then permits use of this easily available cell to monitor ecNOS levels and provides a means to investigate mechanisms involved in controlling NO synthase levels as well as NO synthesis. |
| Databáze: | OpenAIRE |
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