The influence of polyanion molecular weight on polyelectrolyte multilayers at surfaces: protein adsorption and protein–polysaccharide complexation/stripping on natural polysaccharide films on solid supports
| Autor: | David A. Beattie, Marta Krasowska, Sam Karpiniec, James K. Ferri, Natalie L. Benbow, Jessie L. Webber |
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| Přispěvatelé: | Benbow, Natalie L, Webber, Jessie L, Karpiniec, Sam, Krasowska, Marta, Ferri, James K, Beattie, David A |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Chromatography Chemistry Fucoidan General Physics and Astronomy 02 engineering and technology Quartz crystal microbalance Polymer 021001 nanoscience & nanotechnology Polyelectrolyte 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Adsorption Chemical engineering Attenuated total reflection Zeta potential Physical and Theoretical Chemistry 0210 nano-technology Protein adsorption |
| Zdroj: | Physical Chemistry Chemical Physics. 19:23790-23801 |
| ISSN: | 1463-9084 1463-9076 |
| DOI: | 10.1039/c7cp02599h |
| Popis: | Two different fucoidan polymers (unfractionated Fucus vesiculosus fucoidan, and fractionated low molecular weight Fucus vesiculosus fucoidan) have been used to create substrates for protein adsorption studies. Polyelectrolyte multilayers were formed using the fucoidans (polyanions) with chitosan as the corresponding polycation. Multilayer formation was studied using zeta potential measurements, quartz crystal microbalance with dissipation monitoring (QCM-D) and attenuated total reflectance (ATR) FTIR spectroscopy. The formation studies reveal that the low molecular weight (LMW) fucoidan produces a less hydrated multilayer, with a significantly increased adsorbed mass, and with fucoidan as the diffusing species during formation. Protein adsorption studies using bovine serum albumin (BSA) were undertaken for solution conditions designed to mimic biological conditions, and to minimise the role of electrical double layer forces in influencing adsorption. Under these conditions, and as revealed by ATR FTIR spectroscopy, BSA is seen to adsorb less substantially to multilayers formed with the LMW fucoidan, and to cause extraction/stripping of the LMW fucoidan from the multilayer. FTIR spectra reveal that the protein adopts a different conformation when adsorbed to the LMW fucoidan multilayer, both relative to the protein in solution and when adsorbed at the surface of the multilayer formed from unfractionated fucoidan. Refereed/Peer-reviewed |
| Databáze: | OpenAIRE |
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