Phospholipase D protects ECV304 cells against TNFα-induced apoptosis
| Autor: | Michel Lagarde, Annie-France Prigent, Caroline Zeiller, Georges Némoz, Hiba Komati, Hélène Birbes |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Ceramide
Programmed cell death Survival medicine.medical_treatment Biophysics Apoptosis Cycloheximide Biology Ceramides Protective Agents Transfection Biochemistry Cell Line chemistry.chemical_compound Structural Biology Genetics medicine Phospholipase D Humans Tumor necrosis factor α RNA Small Interfering Molecular Biology Tumor Necrosis Factor-alpha Cell Biology Cell biology enzymes and coenzymes (carbohydrates) Cytokine chemistry Cell culture siRNA Tumor necrosis factor alpha lipids (amino acids peptides and proteins) Endothelium Vascular ECV304 cell |
| Zdroj: | FEBS Letters. (26):6224-6232 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2006.10.026 |
| Popis: | Tumor necrosis factor alpha (TNFalpha), a pleiotropic cytokine, activates both apoptotic and pro-survival signals depending on the cell model. Using ECV304 cells, which can be made TNFalpha-sensitive by cycloheximide (CHX) co-treatment, we evaluated the potential roles of ceramide and phospholipase D (PLD) in TNFalpha-induced apoptosis. TNFalpha/CHX induced a robust increase in ceramide levels after 16 h of treatment when cell death was maximal. PLD activity was increased at early time point (1h) whereas both PLD activity and PLD1 protein were strongly decreased after 24h. TNFalpha/CHX-induced cell death was significantly lowered by exogenous bacterial PLD and phoshatidic acid, and in cells overexpressing PLD1. Conversely, cells depleted in PLD proteins by small interference RNA (siRNA) treatment exhibited higher susceptibility to apoptosis. These results show that PLD exerts a protective role against TNFalpha-induced cell death. |
| Databáze: | OpenAIRE |
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