Comparison of chemiluminescent microparticle immunoassay (CMIA) with electrochemiluminescence immunoassay (ECLIA) for Carcinoembryonic antigen (CEA)
| Autor: | Jasmina Smajić, Nafija Serdarevic |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
030213 general clinical medicine
medicine.medical_specialty Nursing (miscellaneous) ECLIA Medicine (miscellaneous) 01 natural sciences Gastroenterology law.invention 03 medical and health sciences CEA 0302 clinical medicine Carcinoembryonic antigen law Internal medicine medicine Electrochemiluminescence In patient Chemiluminescence lcsh:R5-920 medicine.diagnostic_test biology business.industry 010401 analytical chemistry Disease progression 0104 chemical sciences Reference intervals immunoassays Immunoassay biology.protein CMIA lcsh:Medicine (General) business CEA measurement |
| Zdroj: | Journal of Health Sciences, Vol 8, Iss 2 (2018) |
| ISSN: | 1986-8049 2232-7576 |
| DOI: | 10.17532/jhsci.2018.520 |
| Popis: | Introduction: Carcinoembryonic antigen (CEA) is used for monitoring of disease progression and treatment response in cancer patients. Our aim was to compare the performance of chemiluminescent microparticle immunoassay (CMIA) with electrochemiluminescence immunoassay (ECLIA) for CEA. Methods: A total of 115 samples were collected during routine diagnostic, prognostic and therapy monitoring procedures in patients with colorectal and pancreatic cancer. We used ARCHITECT i2000SR and Cobas E601 for CEA analysis in sera samples. Results: The correlation coefficient of 0.984 [95% CI: 0.972 to 0.991] for results obtained on both platforms was observed for CEA≤10 ng/mL group. Moreover, intercept of 0.9027 [95% CI: 0.705 to 1.099] and slope 0.8076 [95% CI: 0.765 to 0.8498] (p < 0.0001) was observed in this group. In CEA >10 ng/mL group we observed slope = 1.1986 [95%CI: 1.1474 to 1.2498] (p < 0.0001), intercept = -11.69 [-17.53 to - 5.84] and correlation coefficient of 0.985 [95% CI: 0.976 to 0.9914]. Mean differences between assays in group ≤10 ng/mL and >10 ng/mL were 0.2066 (95% CI: 0.0019 to 0.4113) and –2.66 (95% CI: -10.10 to 4.76) ng/mL, respectively. Conclusion: Although there were differences, based on 20 days precision tests, overall results showed a good analytical performance and correlation between CEA assays on ARCHITECT i2000SR and Cobas E601 platforms. Reference intervals appropriate for the method of CEA measurement should be used. The standardization and harmonization of serum CEA concentration assays are needed. |
| Databáze: | OpenAIRE |
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