Comparison of two sperm processing techniques for low complexity assisted fertilization: sperm washing followed by swim-up and discontinuous density gradient centrifugation
| Autor: | Lígia F Previato, Paulo C. S. Matheus, Edilberto Araújo, Ligiane A. Machado-Paula, Cassio L. Facio |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Differential centrifugation endocrine system Intrauterine insemination 030219 obstetrics & reproductive medicine Chromatography Density gradient Chemistry urogenital system Sperm washing Semen Sperm Low complexity 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Original Article semen processing Sperm motility swim-up discontinuous concentration gradient |
| Zdroj: | JBRA Assisted Reproduction |
| ISSN: | 1518-0557 1517-5693 |
| Popis: | OBJECTIVE This study aimed to assess and compare sperm motility, concentration, and morphology recovery rates, before and after processing through sperm washing followed by swim-up or discontinuous density gradient centrifugation in normospermic individuals. METHODS Fifty-eight semen samples were used in double intrauterine insemination procedures; 17 samples (group 1) were prepared with sperm washing followed by swim-up, and 41 (group 2) by discontinuous density gradient centrifugation. This prospective non-randomized study assessed seminal parameters before and after semen processing. A dependent t-test was used for the same technique to analyze seminal parameters before and after semen processing; an independent t-test was used to compare the results before and after processing for both techniques. RESULTS The two techniques produced decreases in sample concentration (sperm washing followed by swim-up: P |
| Databáze: | OpenAIRE |
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