31P-NMR study of high-energy phosphorylated compounds metabolism and intracellular pH in the perfused rat heart
| Autor: | Armand Piwnica, E. Fontanarava, M. Bernard, P. Canioni, Philippe Menasché, Robert P. Geyer, P. J. Cozzone |
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| Rok vydání: | 1985 |
| Předmět: |
Male
Magnetic Resonance Spectroscopy Nifedipine Phosphocreatine Physiology Intracellular pH Ischemia chemistry.chemical_element Calcium Biochemistry Phosphates Adenosine Triphosphate Coronary Circulation medicine Animals Chromatography Myocardium Glutamate receptor Antagonist Rats Inbred Strains Metabolism Hydrogen-Ion Concentration medicine.disease Rats Perfusion chemistry Phosphorylation medicine.drug |
| Zdroj: | Archives Internationales de Physiologie et de Biochimie. 93:97-105 |
| ISSN: | 0003-9799 |
| DOI: | 10.3109/13813458509080630 |
| Popis: | Using 31P-NMR and haemodynamical measurements, this work assesses different aspects of myocardial preservation improvement during a global ischaemia, based on a simultaneous and correlated study of high-energy phosphorylated compounds, intracellular pH and left ventricular function. Isolated perfused working rat hearts were subjected to 2 or 3 h of hypothermic ischaemia followed by 30 or 45 min of reperfusion. A study of the influence of pH and buffer used in cardioplegic solutions has demonstrated a better preservation of high-energy phosphates and an improved functional recovery when using a pH 7.0, glutamate - containing solution. Protection provided by cardioplegia can be enhanced by the appropriate use of a fluorocarbon-oxygenated cardioplegic reperfusate. The use of nifedipine, a calcium antagonist, in the cardioplegic solutions, does not provide any additional protection under hypothermic conditions. |
| Databáze: | OpenAIRE |
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