A Secreted Ankyrin-Repeat Protein from Clinical Stenotrophomonas maltophilia Isolates Disrupts Actin Cytoskeletal Structure
| Autor: | Hanlon MacDonald, Mei-Fan Parnes, Logan C. MacDonald, Bryan W. Berger, Emily L. Wong, Lindsey Stretz, Suzanne J. Templer, Sean O’Keefe |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cystic Fibrosis Stenotrophomonas maltophilia Biology Microbiology 03 medical and health sciences Bacterial Proteins Humans Actin-binding protein Cytoskeleton Pathogen Actin Cross Infection Transfection biology.organism_classification Molecular biology Actins In vitro Ankyrin Repeat Anti-Bacterial Agents Actin Cytoskeleton 030104 developmental biology Infectious Diseases Host-Pathogen Interactions biology.protein Ectopic expression Signal Transduction |
| Zdroj: | ACS Infectious Diseases. 2:62-70 |
| ISSN: | 2373-8227 |
| Popis: | Stenotrophomonas maltophilia is an emerging, multidrug-resistant pathogen of increasing importance for the immunocompromised, including cystic fibrosis patients. Despite its significance as an emerging pathogen, relatively little is known regarding the specific factors and mechanisms that contribute to its pathogenicity. We identify and characterize a putative ankyrin-repeat protein (Smlt3054) unique to clinical S. maltophilia isolates that binds F-actin in vitro and co-localizes with actin in transfected HEK293a cells. Smlt3054 is endogenously expressed and secreted from clinical S. maltophilia isolates, but not an environmental isolate (R551-3). The in vitro binding of Smlt3054 to F-actin resulted in a thickening of the filaments as observed by TEM. Ectopic expression of Smlt3054-GFP exhibits strong co-localization with F-actin, with distinct, retrograde F-actin waves specifically associated with Smlt3054 in individual cells as well as formation of dense, internal inclusions at the expense of retrograde F-actin waves. Collectively, our results point to an interaction between Smlt3054 and F-actin. Furthermore, as a potentially secreted protein unique to clinical S. maltophilia isolates, Smlt3054 may serve as a starting point for understanding the mechanisms by which S. maltophilia has become an emergent pathogen. |
| Databáze: | OpenAIRE |
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