Link between Fluorescent Probe Partitioning and Molecular Order of Liquid Ordered-Liquid Disordered Membranes
| Autor: | Jenifer Thewalt, Sherry S.W. Leung |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
1 2-Dipalmitoylphosphatidylcholine Analytical chemistry 010402 general chemistry 01 natural sciences Miscibility 03 medical and health sciences chemistry.chemical_compound Phase (matter) Materials Chemistry Fluorescence microscope Transition Temperature Physical and Theoretical Chemistry Lipid raft Fluorescent Dyes Transition temperature technology industry and agriculture Fluorescence 0104 chemical sciences Surfaces Coatings and Films 030104 developmental biology Membrane Cholesterol chemistry Microscopy Fluorescence Biophysics Phosphatidylcholines lipids (amino acids peptides and proteins) Laurdan |
| Zdroj: | The journal of physical chemistry. B. 121(6) |
| ISSN: | 1520-5207 |
| Popis: | Fluorescence microscopy is an important technique for studying lipid membranes and is increasingly being used for examining liquid ordered-liquid disordered phase coexistence. Liquid–liquid phase coexistence is a phenomenon of biological interest because it led to the lipid raft hypothesis, which postulates the existence of lateral heterogeneities in cell membranes. Observation of membrane heterogeneity relies on differential distribution of fluorescent membrane markers, but this can also modify the phase behavior, complicating the observation. We have used 2H NMR to measure the physical changes to 35:35:30 (mol/mol) DOPC/DPPC-D62/chol membranes introduced by fluorescent probes Laurdan and naphthopyrene. We measured miscibility transition temperature (Tmix) and DPPC-D62 chain order for a range of probe concentrations. We found that up to 0.5 mol% of the equipartitioning probe Laurdan does not influence DPPC-D62 acyl chain order or phase behavior. In contrast, 2.0 mol% Laurdan slightly increases the fracti... |
| Databáze: | OpenAIRE |
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