Occurrence of aminoglycoside-modifying enzymes among isolates of Escherichia coli exhibiting high levels of aminoglycoside resistance isolated from Korean cattle farms
| Autor: | Seung Won Shin, Kuastros Mekonnen Belaynehe, Park Hong-Tae, Han Sang Yoo |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Farms Livestock Tetracycline 030106 microbiology Cattle Diseases Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology 03 medical and health sciences Antibiotic resistance Plasmid Ampicillin Drug Resistance Multiple Bacterial Republic of Korea Genetics medicine Escherichia coli Animals Humans Typing Molecular Biology Escherichia coli Infections Phylogeny Chloramphenicol Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field 030104 developmental biology Aminoglycosides Streptomycin Genes Bacterial Cattle Replicon medicine.drug Plasmids |
| Zdroj: | FEMS microbiology letters. 364(14) |
| ISSN: | 1574-6968 |
| Popis: | This study investigated 247 Escherichia coli isolates collected from four cattle farms to characterize aminoglycoside-modifying enzyme (AME) genes, their plasmid replicons and transferability. Out of 247 isolates a high number of isolates (total 202; 81.78%) were found to be resistant to various antibiotics by disc diffusion. Of the 247 strains, 139 (56.3%) were resistant to streptomycin, and other antibiotic resistances followed as tetracycline (12.15%), ampicillin (7%), chloramphenicol (5.7%) and trimethoprim-sulfamethoxazole (0.8%). Among 247 isolates B1 was the predominant phylogenetic group identified comprising 151 isolates (61.1%), followed by groups A (27.9%), D (7%) and B2 (4%). Out of 139 isolates investigated for AME, 130 (93.5%) isolates carried at least one AME gene. aph3″-1a and aph3″-1b (46%) were the principal genes detected, followed by aac3-IVa (34.5%). ant2″-1a was the least detected gene (2.2%). Nine (6.5%) strains carried no AME genes. Twelve (63.2%) among 19 isolates transferred an AME gene to a recipient and aph3΄-1a was the dominant transferred gene. Transferability mainly occurred via the IncFIB replicon type (52.6%). Pulsed-field gel electrophoresis typing demonstrated a higher degree of diversity with 14 distinct cluster types. This result suggests that commensal microflora from food-producing animals has a tremendous ability to harbor and transfer AME genes, and poses a potential risk by dissemination of resistance to humans through the food chain. |
| Databáze: | OpenAIRE |
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