Resistance to Friend Murine Leukemia Virus Infection Conferred by theFv-4Gene Is Recessive but Appears Dominant from the Effect of the Immune System
| Autor: | Kyoko Higo, Takeshi Ono, Lamin Ta Ya, Akinori Ishimoto, Yasunori Suzuki, Yasumasa Iwatani, Fengmin Zhang, Tomomi Nakahara, Masakazu Tanaka, Kagemasa Kuribayashi, Hiroyuki Sakai |
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| Rok vydání: | 2000 |
| Předmět: |
Immunology
Gene Expression Genes Recessive Biology Microbiology Tacrolimus Flow cytometry Gene product Mice Immune system In vivo Virology Gene expression Genotype medicine Animals Gene Genes Dominant Mice Inbred BALB C Leukemia Experimental medicine.diagnostic_test Friend virus Membrane Proteins biology.organism_classification Immunity Innate Friend murine leukemia virus Tumor Virus Infections Insect Science Pathogenesis and Immunity Immunosuppressive Agents Retroviridae Infections |
| Zdroj: | Journal of Virology. 74:6193-6197 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.74.13.6193-6197.2000 |
| Popis: | Fv-4is a mouse gene that dominantly confers resistance to infection with Friend murine leukemia virus (F-MuLV) (S. Suzuki, Jpn. J. Exp. Med. 45:473–478, 1975). However, the resistance caused byFv-4is recessive in nude mice, which suggests that immunological effects play important roles in this resistance in vivo (K. Higo, Y. Kubo, Y. Iwatani, T. Ono, M. Maeda, H. Hiai, T. Masuda, K. Kuribayashi, F. Zhang, T. Lamin, A. Adachi, and A. Ishimoto, J. Virol. 71:750–754, 1997). To determine the immunological effect on the resistance in vivo, we infected immunologically immature newborn mice homozygous (Fv-4r/r) and heterozygous (Fv-4r/−) forFv-4. Although theFv-4r/rmice showed complete resistance to F-MuLV whether infected neonatally or as adolescents, theFv-4r/−mice showed high sensitivity to viral proliferation and disease induction when infected as newborns but complete resistance when infected as adolescent mice. To confirm the immunological effect on the resistance in adolescent mice with theFv-4r/randFv-4r/−genotypes, we examined the effect of an immunosuppressant drug, FK506, on the resistance. The mice with theFv-4r/rgenotype treated with FK506 still showed resistance, but the mice with theFv-4r/−genotype became highly sensitive to F-MuLV infection. Flow cytometric analysis to detect theFv-4gene product showed that theFv-4gene product was expressed on the cells from newborn and adolescent mice. TheFv-4gene product was also detected on the cells from the FK506-treated mice as well as on those from untreated mice. However, a quantitative difference in the gene product between the cells with theFv-4r/randFv-4r/−genotypes was detected by indirect staining for flow cytometry. These results show that the resistance to F-MuLV infection conferred by theFv-4gene is originally recessive, but it looks dominant in adolescent mice mainly because of the effect of the immune system. |
| Databáze: | OpenAIRE |
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