Host-derived circular RNAs display proviral activities in Hepatitis C virus-infected cells
| Autor: | Pakpoom Boonchuen, Juana Díez, Peter Sarnow, Tzu-Chun Chen, René Böttcher, Sebastian Kadener, Qian M. Cao, Gemma Pérez-Vilaró, Kunlaya Somboonwiwat, Marc Talló-Parra |
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| Rok vydání: | 2020 |
| Předmět: |
RNA viruses
Small interfering RNA Hydrolases viruses Viral pathogenesis Artificial Gene Amplification and Extension Hepacivirus Virus Replication medicine.disease_cause Biochemistry Polymerase Chain Reaction DEAD-box RNA Helicases Fetge Proviruses Biology (General) Pathology and laboratory medicine 0303 health sciences Hepatitis C virus Liver Neoplasms 030302 biochemistry & molecular biology Medical microbiology Hepatitis C Enzymes Cell biology Nucleic acids Viruses RNA Viral Pathogens Oxidoreductases Luciferase Research Article Carcinoma Hepatocellular Nucleases QH301-705.5 Immunology Biology Transfection Research and Analysis Methods Microbiology Viral Proteins 03 medical and health sciences Ribonucleases Circular RNA Virology DNA-binding proteins microRNA Genetics medicine Humans Non-coding RNA Molecular Biology Techniques Molecular Biology 030304 developmental biology Medicine and health sciences Innate immune system Biology and life sciences Flaviviruses Gene Expression Profiling Organisms Viral pathogens Proteins RNA RNA Circular Dengue Virus RC581-607 Hepatitis viruses Viral Replication Nonsense Mediated mRNA Decay Gene regulation Microbial pathogens Viral replication Eukaryotic Initiation Factor-4A Enzymology Virus de l'hepatitis C Parasitology Gene expression Immunologic diseases. Allergy Genètica |
| Zdroj: | PLoS Pathogens, Vol 16, Iss 8, p e1008346 (2020) PLoS Pathogens |
| ISSN: | 1553-7374 |
| DOI: | 10.1371/journal.ppat.1008346 |
| Popis: | Viruses subvert macromolecular pathways in infected host cells to aid in viral gene amplification or to counteract innate immune responses. Roles for host-encoded, noncoding RNAs, including microRNAs, have been found to provide pro- and anti-viral functions. Recently, circular RNAs (circRNAs), that are generated by a nuclear back-splicing mechanism of pre-mRNAs, have been implicated to have roles in DNA virus-infected cells. This study examines the circular RNA landscape in uninfected and hepatitis C virus (HCV)-infected liver cells. Results showed that the abundances of distinct classes of circRNAs were up-regulated or down-regulated in infected cells. Identified circRNAs displayed pro-viral effects. One particular up-regulated circRNA, circPSD3, displayed a very pronounced effect on viral RNA abundances in both hepatitis C virus- and Dengue virus-infected cells. Though circPSD3 has been shown to bind factor eIF4A3 that modulates the cellular nonsense-mediated decay (NMD) pathway, circPSD3 regulates RNA amplification in a pro-viral manner at a post-translational step, while eIF4A3 exhibits the anti-viral property of the NMD pathway. Findings from the global analyses of the circular RNA landscape argue that pro-, and likely, anti-viral functions are executed by circRNAs that modulate viral gene expression as well as host pathways. Because of their long half-lives, circRNAs likely play hitherto unknown, important roles in viral pathogenesis. Author summary Usually, cells are infected by one or a few virus particles that carry genomes with limited expression capacity. Thus, the expression of viral genomes has to compete with a sea of cellular components that aid in viral translation, replication and virion production. Depending on their lifestyle, viruses have evolved to avoid or to subvert cellular pathways, especially those that display anti-viral functions. Host-derived circular RNA molecules have recently been discovered in the cytoplasm of cells, although, as-of yet, few functions have been assigned to them. Here, we describe alterations in the circular RNA landscape in hepatitis C virus-infected liver cells. Up-regulated and down-regulated circular RNAs were identified, and three of the upregulated RNAs were shown to promote HCV infection. One of them, circPSD3, inhibited viral RNA abundance at a post-translational step. Because circular RNAs are more stable than linear RNAs, they may have important functions during viral infection, dictating the outcomes of innate immune responses and viral pathogenesis. |
| Databáze: | OpenAIRE |
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