Nuclear factor of activated T cells negatively regulates expression of the tumor necrosis factor receptor-related 2 gene in T cells
| Autor: | Ok Ju Sul, Beverly H. Koller, Hye Young Hur, Anne M. Latour, Choon Soo Jeong, Jung Sook Kwak, Byoung S. Kwon, Woon-Ki Kim |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
CD4-Positive T-Lymphocytes
T-Lymphocytes Clinical Biochemistry Molecular Sequence Data Down-Regulation Biology Biochemistry Mice Immune system Downregulation and upregulation Gene expression Animals Molecular Biology Cells Cultured Regulation of gene expression Reporter gene Base Sequence NFATC Transcription Factors NFAT Molecular biology Mice Inbred C57BL Molecular Medicine Tumor necrosis factor alpha Original Article Receptors Tumor Necrosis Factor Member 14 |
| DOI: | 10.17615/3549-kn98 |
| Popis: | Tumor necrosis factor receptor-related 2 (TR2, HVEM or TNFRSF-14) plays an important role in immune responses, however, the mechanisms regulating its expression are unclear. To understand the control of TR2 gene expression, we studied the upstream region of the gene. Gel supershift assays revealed inducible binding of nuclear factor of activated T cells (NFAT) to a putative NFAT site within the TR2 promoter. Furthermore, cotransfection of a dominant negative NFAT construct, or siRNA for NFAT, resulted in increased expression of a TR2 reporter gene. Our findings demonstrate that NFAT negatively regulates TR2 expression in activated T cells. |
| Databáze: | OpenAIRE |
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