Evaluation of active and passive transport processes in corneas extracted from preserved rabbit eyes
| Autor: | Soumyajit Majumdar, Ramesh Srirangam, Tushar Hingorani |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Amino Acid Transport Systems genetic structures Passive transport medicine.medical_treatment Organ Preservation Solutions Biological Transport Active Pharmaceutical Science Biology Article Cornea Ophthalmology Electric Impedance medicine Animals Transcellular Saline Lagomorpha Esterases Biological Transport Organ Preservation biology.organism_classification eye diseases In vitro medicine.anatomical_structure Biochemistry Permeability (electromagnetism) Paracellular transport Models Animal Rabbits sense organs |
| Zdroj: | Journal of Pharmaceutical Sciences. 99:1921-1930 |
| ISSN: | 0022-3549 |
| DOI: | 10.1002/jps.21979 |
| Popis: | In vitro transcorneal permeability studies are an important screening tool in drug development. The objective of this research is to examine the feasibility of using corneas isolated from preserved rabbit eyes as a model for permeability evaluation. Eyes from male New Zealand White rabbits were used immediately or were stored overnight in phosphate-buffered saline (PBS) or Hanks balanced salt solution (HBSS) over wet ice. Integrity of isolated corneas was evaluated by measuring the TEER and by determining the permeability of paracellular and transcellular markers. Active transport was assessed by measuring transcorneal permeability of selected amino acids. Esterase activity was estimated using p-nitrophenyl assay. In all cases, corneas from freshly enucleated eyes were compared to those isolated from the day-old preserved eyes. Transcellular and paracellular passive diffusion was not affected by the storage medium and observed to be similar in the fresh and preserved eye models. However, amino acid transporters demonstrated lower functional activity in corneas excised from eyes preserved in PBS. Moreover, preserved eyes displayed almost 1.5-fold lower esterase activity in the corneal tissue. Thus, corneas isolated from day-old eyes, preserved in HBSS, closely mimics freshly excised rabbit corneas in terms of both active and passive transport characteristics but possesses slightly reduced enzymatic activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text