Endothelial Cell Autonomous Role of Akt1
| Autor: | Monica Y. Lee, Michael Simons, William C. Sessa, David J. Vinyard, Ana Gamez-Mendez, Jan R. Kraehling, Themis R. Kyriakides, Gary W. Brudvig, Zhenwu Zhuang, Heino Velazquez, Jiasheng Zhang |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Nitric Oxide Synthase Type III Angiogenesis Ischemia Neovascularization Physiologic Aorta Thoracic Blood Pressure Nitric Oxide Article 03 medical and health sciences Enos Animals Medicine Phosphorylation Muscle Skeletal Protein kinase B PI3K/AKT/mTOR pathway Mice Knockout biology business.industry Endothelial Cells medicine.disease biology.organism_classification Hindlimb Cell biology Endothelial stem cell Disease Models Animal 030104 developmental biology Regional Blood Flow Vasoconstriction embryonic structures Arteriogenesis Cardiology and Cardiovascular Medicine business Proto-Oncogene Proteins c-akt Blood Flow Velocity Homeostasis Signal Transduction |
| Zdroj: | Arteriosclerosis, Thrombosis, and Vascular Biology. 38:870-879 |
| ISSN: | 1524-4636 1079-5642 |
| DOI: | 10.1161/atvbaha.118.310748 |
| Popis: | Objective— The importance of PI3K/Akt signaling in the vasculature has been demonstrated in several models, as global loss of Akt1 results in impaired postnatal ischemia- and VEGF-induced angiogenesis. The ubiquitous expression of Akt1, however, raises the possibility of cell-type–dependent Akt1-driven actions, thereby necessitating tissue-specific characterization. Approach and Results— Herein, we used an inducible, endothelial-specific Akt1-deleted adult mouse model (Akt1iECKO) to characterize the endothelial cell autonomous functions of Akt1 in the vascular system. Endothelial-targeted ablation of Akt1 reduces eNOS (endothelial nitric oxide synthase) phosphorylation and promotes both increased vascular contractility in isolated vessels and elevated diastolic blood pressures throughout the diurnal cycle in vivo. Furthermore, Akt1iECKO mice subject to the hindlimb ischemia model display impaired blood flow and decreased arteriogenesis. Conclusions— Endothelial Akt1 signaling is necessary for ischemic resolution post-injury and likely reflects the consequence of NO insufficiency critical for vascular repair. |
| Databáze: | OpenAIRE |
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