Development of a Novel Orthogonal Double Gradient for High-Throughput Screening of Mesenchymal Stem Cells-Materials Interaction
| Autor: | Lu Ge, Qihui Zhou, Patrick van Rijn, Carlos F. Guimarães, Liangliang Yang, Philipp T. Kühn |
|---|---|
| Přispěvatelé: | Nanotechnology and Biophysics in Medicine (NANOBIOMED), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science animal structures wettability Biointerface 02 engineering and technology Matrix (biology) ADHESION high-throughput screening orthogonal double gradient Contact angle 03 medical and health sciences SUBSTRATE MICROENVIRONMENTS medicine BIOMATERIALS biology Mechanical Engineering Mesenchymal stem cell POLYMER SURFACES Stiffness STIFFNESS Adhesion Vinculin CHEMICAL GRADIENTS 021001 nanoscience & nanotechnology stem cell 030104 developmental biology DIFFERENTIATION Mechanics of Materials biology.protein Wetting CONTACT GUIDANCE medicine.symptom 0210 nano-technology MATRIX Biomedical engineering |
| Zdroj: | Advanced Materials Interfaces, 5(18):1800504. Wiley |
| ISSN: | 2196-7350 |
| DOI: | 10.1002/admi.201800504 |
| Popis: | Surface gradients provide a powerful platform to accelerate multiscale design by efficiently studying of material-cell interactions to ultimately enhance function of synthetic clinical biomaterials. Herein, a novel orthogonal double gradient is reported in which surface stiffness and wettability vary independently and continuously in perpendicular directions, providing unique combinations of stiffness and wettability over a broad range (stiffness: 6-89 MPa; water contact angle: 29 degrees-90 degrees). It is found that mesenchymal stem cell behavior is nonlinearly regulated by surface stiffness and wettability. These combined stiffness and wettability properties of a material significantly affect stem cell adhesion, spreading, nucleus size, and vinculin expression. This high-throughput screening system enables elucidation of the relationships between biointerface properties and biological behavior, and thereby serves a potential tool for accelerating the development of high-performance biomaterials. |
| Databáze: | OpenAIRE |
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