Quantification of mast cells in the uterine cervix of women infected with human immunodeficiency virus
| Autor: | Ana Karina Marques Salge, Karina Machado Siqueira, Janaína Valadares Guimarães, Fabiana Bettanin Costa, Wanessa Machado Andrade, Vicente de Paula Antunes Texeira, Eneida Franco Vêncio |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Tryptase Cell Count HIV Infections Cervix Uteri Azure Stains Giemsa stain Pathology and Forensic Medicine Chymases medicine Humans Mast Cells Cervix biology Chymase General Medicine Middle Aged Mast cell Immunohistochemistry Staining medicine.anatomical_structure biology.protein HIV-1 Female Tryptases Antibody Biomarkers Mastocytosis |
| Zdroj: | Annals of diagnostic pathology. 15(5) |
| ISSN: | 1532-8198 |
| Popis: | The objective of the study was to identify mastocytosis in the chorionic epithelium of the uterine cervix in HIV-infected and non–HIV-infected women in autopsy specimens using histochemistry and immunohistochemistry techniques. Sixteen cervical tissue specimens were collected, of which 10 (62.50%) were from HIV-infected women. Histochemical and immunohistochemical techniques were used to evaluate mast cell density using Giemsa stain and anti-mast cell tryptase and anti-mast cell chymase antibodies, respectively. The study of the sheets and counting of mast cells with blue (Giemsa) or brown staining (anti-mast cell tryptase or chymase antibodies) were performed by 3 examiners, and 10 consecutive fields were examined under a light microscope at 400× magnification. A significant difference was found in mast cell density in the chorionic epithelium of the cervix in HIV-infected compared with non–HIV-infected women. The present study may contribute to the characterization of genital mucosa abnormalities and help better understand the potential role of mast cells in HIV-infected women. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect