Astacin-like metalloproteases are a gene family of toxins present in the venom of different species of the brown spider (genus Loxosceles)
| Autor: | Silvio Sanches Veiga, Waldemiro Gremski, Luiza Helena Gremski, Katia C. Barbaro, Gabriel Otto Meissner, Dilza Trevisan-Silva, Oldemir C. Mangili, Olga Meiri Chaim, Andrea Senff-Ribeiro, Rafael Bertoni da Silveira |
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| Rok vydání: | 2010 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Spider Venoms Venom Cross Reactions Biology medicine.disease_cause complex mixtures Biochemistry Antibodies Mice Complementary DNA medicine Animals Humans Gene family Amino Acid Sequence RNA Messenger Cloning Molecular Gene chemistry.chemical_classification Base Sequence Phosphoric Diester Hydrolases cDNA library Toxin Metalloendopeptidases Spiders Sequence Analysis DNA General Medicine Molecular biology Amino acid chemistry Metalloproteases Gelatin Astacin Sequence Alignment Phenanthrolines |
| Zdroj: | Biochimie. 92:21-32 |
| ISSN: | 0300-9084 |
| DOI: | 10.1016/j.biochi.2009.10.003 |
| Popis: | Brown spiders have a worldwide distribution, and their venom has a complex composition containing many different molecules. Herein, we report the existence of a family of astacin-like metalloprotease toxins in Loxosceles intermedia venom, as well as in the venom of different species of Loxosceles. Using a cDNA library from the L. intermedia venom gland, we cloned two novel cDNAs encoding astacin-like metalloprotease toxins, LALP2 and LALP3. Using an anti-serum against the previously described astacin-like toxin in L. intermedia venom (LALP1), we detected the presence of immunologically-related toxins in the venoms of L. intermedia, Loxosceles laeta, and Loxosceles gaucho. Zymographic experiments showed gelatinolytic activity of crude venoms of L. intermedia, L. laeta, and L. gaucho (which could be inhibited by the divalent metal chelator 1,10-phenanthroline) at electrophoretic mobilities identical to those reported for immunological cross-reactivity. Moreover, mRNAs extracted from L. laeta and L. gaucho venom glands were screened for astacin-like metalloproteases, and cDNAs obtained using LALP1-specific primers were sequenced, and their deduced amino acid sequences confirmed they were members of the astacin family with the family signatures (HEXXHXXGXXHE and MXY), LALP4 and LALP5, respectively. Sequence comparison of deduced amino acid sequences revealed that LALP2, LALP3, LALP4, and LALP5 are related to the astacin family. This study identified the existence of gene family of astacin-like toxins in the venoms of brown spiders and raises the possibility that these molecules are involved in the deleterious effects triggered by the venom. |
| Databáze: | OpenAIRE |
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