Retrospective evaluation of the Du Pont radioimmunoassay kit for detection of Legionella pneumophila serogroup 1 antigenuria in humans
| Autor: | M E Aguero-Rosenfeld, Paul H. Edelstein |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Microbiology (medical)
Legionella Radioimmunoassay Bacteriuria Urine Legionella pneumophila Microbiology Predictive Value of Tests medicine Humans Legionella pneumophila Serogroup 1 Retrospective Studies Antigens Bacterial biology business.industry bacterial infections and mycoses medicine.disease biology.organism_classification Pneumonia Legionnaires' disease Reagent Kits Diagnostic Legionnaires' Disease business Research Article |
| Zdroj: | Journal of Clinical Microbiology. 26:1775-1778 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | We used the Du Pont radioimmunoassay kit for soluble Legionella pneumophila serogroup 1 antigenuria (Du Pont Co., Wilmington, Del.) to test 422 urine samples from patients with and without Legionnaires disease (LD). The urine specimens were collected from 23 patients with culture-proven LD and from 346 patients without LD. L. pneumophila serogroup 1 was isolated from 14 patients with culture-proven LD, and other L. pneumophila serogroups or other Legionella species were isolated from 9 patients; 58 urine specimens were tested from these 23 patients. The non-LD group was composed of 75 bacteremic patients (35 gram-negative and 40 gram-positive bacteremias), 7 patients with candidemia, 48 patients with non-LD pneumonia, 90 patients with gram-negative bacteriuria (greater than 10(5) CFU/ml), 23 patients with gram-positive bacteriuria (greater than 10(5) CFU/ml), 14 patients with candiduria (greater than 10(5) CFU/ml), and 89 outpatients with negative urine cultures. All tests were performed in duplicate, including positive and negative controls. Sample results with values greater than or equal to 3.0 times those of the negative controls were considered positive for L. pneumophila serogroup 1 antigenuria. The average sample-to-negative ratios were 19.1 for the L. pneumophila serogroup 1 specimens, and 1.0 for both the non-serogroup 1 legionella group and the non-LD specimens. All but one of the patients who were culture positive for L. pneumophila serogroup 1 had at least one specimen positive for serogroup 1 antigenuria; none of the non-L. pneumophila serogroup 1 patients had a positive urine test. The test was highly specific (100%) and sensitive (93%) for the detection of L. pneumophila serogroup 1 antigenuria. Concentrations of urine by vacuum evaporation increased test sensitivity without apparently affecting specificity. |
| Databáze: | OpenAIRE |
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