Implications of matrix effects in ultra-fast gradient or fast isocratic liquid chromatography with mass spectrometry in drug discovery
| Autor: | Philip R. Tiller, Leslie A. Romanyshyn |
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| Rok vydání: | 2001 |
| Předmět: |
Quality Control
Analyte Chromatography Resolution (mass spectrometry) Chemistry Drug discovery Organic Chemistry Analytical chemistry Drugs Investigational Urine Mass spectrometry High-performance liquid chromatography Mass Spectrometry Rats Specimen Handling Analytical Chemistry Matrix (chemical analysis) Blood Animals Bile Ultra fast Sample preparation Chromatography High Pressure Liquid Spectroscopy Chromatography Liquid |
| Zdroj: | Rapid Communications in Mass Spectrometry. 16:92-98 |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/rcm.544 |
| Popis: | In the analysis of biological samples it is important to reduce the risk of interferences from the matrix itself, other analytes, the dosing vehicle (commonly PEG), and from the MS/MS transitions used for the analysis. Rapid analysis is essential for drug discovery, and even though the requirements for separation may be minimized for speed, the integrity of the analysis is still dependent on the separation. This paper focuses on the potential for interferences from various endogenous and exogenous matrix components commonly encountered in quantitation of analytes and their metabolites from biological matrices. We demonstrate that neither high organic isocratic nor ballistic gradient ultra-fast HPLC show a clearly defined advantage in regards to complex biological matrices. The critical factor in the resolution of matrix interferences still remains in sample preparation. |
| Databáze: | OpenAIRE |
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