Adenovirus Replication Cycle Disruption from Exposure to Polychromatic Ultraviolet Irradiation
| Autor: | Kelley Gonçalves, Bernardo Vazquez-Bravo, Joanna L. Shisler, Benito J. Mariñas |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
DNA Replication
Ultraviolet Rays Adenoviridae Infections viruses 0208 environmental biotechnology 02 engineering and technology 010501 environmental sciences Virus Replication medicine.disease_cause 01 natural sciences Genome Adenoviridae medicine Humans Environmental Chemistry Irradiation 0105 earth and related environmental sciences Virus quantification Chemistry Adenoviruses Human General Chemistry Replication cycle Molecular biology Reverse transcriptase 020801 environmental engineering Real-time polymerase chain reaction Capsid DNA Viral Ultraviolet |
| Zdroj: | Environmental Science & Technology. 52:3652-3659 |
| ISSN: | 1520-5851 0013-936X |
| DOI: | 10.1021/acs.est.7b06082 |
| Popis: | Polychromatic ultraviolet (UV) light with bandwidth of 20 nm and peak emission centered at 224, 254, or 280 nm (UV224, UV254, and UV280, respectively) were used to inactivate human adenovirus type 2 (HAdV-2). Quantitative polymerase chain reaction (qPCR) and reverse transcriptase qPCR assays were used to elucidate the step in the HAdV-2 replication cycle that was disrupted after UV exposure. UV treatment at any of the wavelengths analyzed did not inhibit association of HAdV-2 to the host cells even after exposure to a fluence (UV dose) that would produce a virus inactivation efficiency, measured by plaque assay, greater than 99.99%. In contrast, UV irradiation at all three peak emissions disrupted early E1A gene transcription and viral DNA replication, but different mechanisms appeared to be dominating such disruptions. UV224 seemed to have little effect on the integrity of the viral genome but produced a structural transformation of the viral capsid that may inhibit the delivery of viral genome into the ... |
| Databáze: | OpenAIRE |
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