Development of a novel method for the strengthening and toughening of irradiation-sterilized bone allografts
| Autor: | Timothy E. Burrow, Mitchell Woodside, Gagan Minhas, David S. Josey, Xing Ze Lu, Thomas L. Willett, Marc D. Grynpas, Tarik Attia |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Free Radicals Ribose Biomedical Engineering Human bone Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ultimate tensile strength medicine Humans Femur Irradiation Pentosidine Aged 030222 orthopedics Transplantation Bone Transplantation Sterilization Cell Biology Middle Aged Allografts Biocompatible material Toughening Biomechanical Phenomena Surgery 030104 developmental biology medicine.anatomical_structure chemistry Gamma Rays Cortical bone Collagen Stress Mechanical Biomedical engineering |
| Zdroj: | Cell and Tissue Banking. 18:323-334 |
| ISSN: | 1573-6814 1389-9333 |
| DOI: | 10.1007/s10561-017-9634-5 |
| Popis: | Reconstruction of large skeletal defects is a significant and challenging issue. Bone allografts are often used for such reconstructions. However, sterilizing bone allografts by using γ-irradiation, damages collagen and causes the bone to become weak, brittle and less fatigue resistant. In a previous study, we successfully protected the mechanical properties of human cortical bone by conducting a pre-treatment with ribose, a natural and biocompatible agent. This study focuses on examining possible mechanisms by which ribose might protect the bone. We examined the mechanical properties, crosslinking, connectivity and free radical scavenging potentials of the ribose treatment. Human cortical bone beams were treated with varying concentration of ribose (0.06–1.2 M) and γ-irradiation before testing them in 3-point bending. The connectivity and amounts of crosslinking were determined with Hydrothermal-Isometric-Tension testing and High-Performance-Liquid-Chromatography, respectively. The free radical content was measured using Electron Paramagnetic Resonance. Ribose pre-treatment improved the mechanical properties of irradiation sterilized human bone in a pre-treatment concentration-dependent manner. The 1.2 M pre-treatment provided >100% of ultimate strength of normal controls and protected 76% of the work-to-fracture (toughness) lost in the irradiated controls. Similarly, the ribose pre-treatment improved the thermo-mechanical properties of irradiation-sterilized human bone collagen in a concentration-dependent manner. Greater free radical content and pentosidine content were modified in the ribose treated bone. This study shows that the mechanical properties of irradiation-sterilized cortical bone allografts can be protected by incubating the bone in a ribose solution prior to irradiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink