A cis-acting element that directs the activity of the murine methylation modifier locusSsm1
| Autor: | Peter Engler, Lynn Doglio, Ursula Storb, Grazyna Bozek |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
Ribosomal Proteins Saccharomyces cerevisiae Proteins Transgene Mice Transgenic Locus (genetics) Biology X-inactivation Fungal Proteins Mice Animals Transgenes RNA-Directed DNA Methylation Genetics Mice Inbred BALB C Multidisciplinary Chromosome Mapping Methylation DNA Methylation Biological Sciences Position-effect variegation Pedigree Mice Inbred C57BL CpG site DNA methylation Female Gene Deletion |
| Zdroj: | Proceedings of the National Academy of Sciences. 95:10763-10768 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.95.18.10763 |
| Popis: | Silencing of chromosomal domains has been described in diverse systems such as position effect variegation in insects, silencing near yeast telomeres, and mammalian X chromosome inactivation. In mammals, silencing is associated with methylation at CpG dinucleotides, but little is known about how methylation patterns are established or altered during development. We previously described a strain-specific modifier locus,Ssm1, that controls the methylation of a complex transgene. In this study we address the questions of the nature ofSsm1’s targets and whether its effect extends into adjacent sequences. By examining the inheritance of methylation patterns in a series of mice harboring deletion derivatives of the original transgene, we have identified a discrete segment, derived from thegptgene ofEscherichia coli, that is a major determinant forSsm1-mediated methylation. Methylation analysis of sequences adjacent to a transgenic target indicates that the influence of this modifier extends into the surrounding chromosome in a strain-dependent fashion. Implications for the mechanism ofSsm1action are discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|