The role of hyaluronic acid inclusion on the energetics of encapsulation and release of a protein molecule from chitosan-based nanoparticles
| Autor: | Sonia Al-Qadi, Carmen Remuñán-López, Manuel Alatorre-Meda, Manuel Martín-Pastor, Pablo Taboada |
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| Rok vydání: | 2016 |
| Předmět: |
Steric effects
Magnetic Resonance Spectroscopy Drug Compounding Nanoparticle 02 engineering and technology 010402 general chemistry Binding Competitive 01 natural sciences Chitosan chemistry.chemical_compound Drug Delivery Systems Colloid and Surface Chemistry Differential scanning calorimetry Microscopy Electron Transmission Hypoglycemic Agents Insulin Molecule Organic chemistry Hyaluronic Acid Physical and Theoretical Chemistry Drug Carriers Calorimetry Differential Scanning Isothermal titration calorimetry Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology Polyelectrolyte 0104 chemical sciences Drug Liberation chemistry Drug delivery Biophysics Nanoparticles Thermodynamics 0210 nano-technology Algorithms Protein Binding Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 141:223-232 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2016.01.029 |
| Popis: | The synergistic effects of the polysaccharides chitosan (CS) and hyaluronic acid (HA) formulated into hybrid nanoparticles are promising for drug delivery. In the present work, we performed a detailed analysis of the molecular interactions involved in the TPP-assisted ionotropic gelation of CS hybrid nanoparticles with the objective of investigating the impact of HA inclusion on the particle formulation and on the in vitro release of insulin (INS) as a protein cargo. To do that, an in-depth thermodynamic study was carried out by isothermal titration calorimetry (ITC), nuclear magnetic resonance (NMR) and differential scanning calorimetry (DSC) techniques. Such analysis allowed us to elucidate the type and extent of interactions established by INS within the hybrid nanoparticles and to get further knowledge on the nature of its release mechanism in vitro. Overall, INS release from the CS nanoparticles was thermodynamically driven, and when including HA a weaker INS binding to the nanoparticles, hence, a faster release rate in vitro were observed. As a negative polyelectrolyte, HA might have sterically blocked the activated sites of CS, such as the amino groups, through chain entanglement, thereby, attenuating the competitive binding interactions of INS. As a consequence, INS might have experienced a spatial exclusion onto the surface of the hybrid nanoparticles to a greater extent which, in turn, would explain its initial abrupt release. |
| Databáze: | OpenAIRE |
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