Identification and analysis of olfactory genes in Dioryctria abietella based on the antennal transcriptome
| Autor: | Jia Yu, De-Fu Chi, Ya Xing, Muhammad Adeel, Wariya Thanasirungkul, Asad Aslam |
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| Rok vydání: | 2021 |
| Předmět: |
Arthropod Antennae
Male Physiology Genes Insect Biology Receptors Odorant Dioryctria abietella Biochemistry Lepidoptera genitalia Transcriptome 03 medical and health sciences Genetics Animals Mating Molecular Biology Phylogeny 030304 developmental biology 0303 health sciences Gene Expression Profiling fungi 030305 genetics & heredity biology.organism_classification Lepidoptera Smell Membrane protein Evolutionary biology Sex pheromone Odorant-binding protein biology.protein Insect Proteins Female PEST analysis |
| Zdroj: | Comparative Biochemistry and Physiology Part D: Genomics and Proteomics. 38:100814 |
| ISSN: | 1744-117X |
| Popis: | The coneworm Dioryctria abietella (Lepidoptera: Pyralidae) is an economy devastating pest that infests many valuable conifer species in the Holarctic regions, such as Pinus koraiensis Siebold and Zucc. The chemosensory system plays a crucial role in the mating, foraging, and ovipositing of this pest, and therefore it is desirable to identify chemosensory molecules for pest control. However, little is known at molecular level about the olfactory mechanisms in D. abietella. In the present study, we first established antennal transcriptomes of D. abietella and identified 132 putative chemosensory genes, including 15 odorant-binding proteins, 18 chemosensory proteins, 65 odorant receptors, 5 sensory neuron membrane proteins, 24 ionotropic receptors, and 5 gustatory receptors. In addition, phylogenetic trees were constructed for chemosensory genes to investigate the orthologs between D. abietella and other species of insects. Furthermore, we also compared the patterns of motifs between OBPs and CSPs using MEME. Additionally, we observed that most of DabiOBPs and DabiCSPs had the antenna-biased expression by quantitative real-time PCR (RT-qPCR), and there was a higher expression of DabiPBP1 and DabiPBP2 in male antennae than in female antennae. The binding sites of DabiPBPs (DabiPBP1, DabiPBP2) and DabiPRs (DabiOR19, DabiOR31) to the sex pheromone were predicted well by three-dimensional docking structure modelling and molecular docking. Our finding supplied a foundation for further research on the binding process of OBPs or CSPs and sensing process of ORs, SNMPs, IRs or GRs in D. abietella. |
| Databáze: | OpenAIRE |
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