Endometrial expression of estrogen and progesterone receptors in non-cyclic mares treated only with long-acting progesterone
| Autor: | J. A. C. Lourenção, Cezinande de Meira, E. S. M. Silva, R.S. Rossi, José P. Oliveira-Filho, S. C. Fritsch, Luciana F.S. Maciel |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), UNITRI- Centro Universitário do Triângulo |
| Rok vydání: | 2018 |
| Předmět: |
040301 veterinary sciences
medicine.drug_class Biology Anestrus 0403 veterinary science Andrology Endometrium Food Animals Gene expression Progesterone receptor medicine Animals Horses RNA Messenger Small Animals Receptor Progesterone Equine Gene Expression Profiling 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences Embryo Transfer Immunohistochemistry 040201 dairy & animal science Embryo transfer Hormonal protocols Receptors Estrogen Estrogen Female Animal Science and Zoology Receptors Progesterone Estrogen receptor alpha Immunostaining |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0093-691X |
| DOI: | 10.1016/j.theriogenology.2017.11.037 |
| Popis: | Made available in DSpace on 2018-12-11T17:18:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-03-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Administration of progesterone (P4) after estradiol is usually performed to prepare non-cyclic mares as embryo recipients. However, there are successful pregnancy reports after embryo transfer in non-cyclic mares treated only with progestins. The objective of this study was to evaluate endometrial gene expression and immunostaining for estrogen receptor alpha (ERα), beta (ERβ) and progesterone receptor (PR) in seasonal anestrous mares treated with long acting P4 (LA P4). Endometrial tissue from eight seasonal anestrous mares was collected immediately before administration of 1.5 g of LA P4 and five days after. The receptors protein expression was evaluated by immunohistochemistry and the percentage of the immunostained area was determined by ImageJ software. Transcripts abundance for ERα ERβ and PR were determined by RT-qPCR. Blood samples were collected daily to measure plasma P4 concentrations. Protein expression for ERα was greater (p < 0.05) after LA P4 administration, although gene expression was not affected by treatment (p > 0.05). There was no difference for ERβ protein expression (p = 0.07) and ERβ gene expression was reduced (p < 0.05) after treatment. Gene and protein expression for PR was not altered (p > 0.05). In conclusion, endometrial PR and ERα expression patterns after LA P4 administration were similar to those previously found in protocols using estradiol prior to LA P4 to prepare non-cyclic mares as embryo recipients. UNESP-Univ. Estadual Paulista Campus de Botucatu Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNITRI- Centro Universitário do Triângulo UNESP-Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Clinical Science School of Veterinary Medicine and Animal Science UNESP- Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Science UNESP-Univ. Estadual Paulista Campus de Botucatu Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science UNESP-Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Clinical Science School of Veterinary Medicine and Animal Science UNESP- Univ. Estadual Paulista Campus de Botucatu Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Science FAPESP: 2012/00298-7 |
| Databáze: | OpenAIRE |
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