Phylloseptin-1 is Leishmanicidal for Amastigotes of Leishmania amazonensis Inside Infected Macrophages
| Autor: | Gustavo Adolfo Sierra Romero, Tatiana Karla dos Santos Borges, José Roberto S. A. Leite, Luciana M. Leite, Daniel C. Moreira, Selma A S Kuckelhaus, Maura V. Prates, Daniela Sant’Ana de Aquino, Carlos S. Kückelhaus, Maria Imaculada Muniz-Junqueira, Carlos Bloch |
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| Přispěvatelé: | SELMA A. S. KÜCKELHAUS, UNB, DANIELA SANT'ANA DE AQUINO, UNB, TATIANA K. BORGES, UNB, DANIEL C. MOREIRA, UNB, LUCIANA DE MAGALHÃES LEITE, UNB, MARIA IMACULADA MUNIZ-JUNQUEIRA, UNB, CARLOS S. KÜCKELHAUS, UNB, GUSTAVO A. SIERRA ROMERO, UNB, MAURA VIANNA PRATES, Cenargen, CARLOS BLOCH JUNIOR, Cenargen, JOSÉ ROBERTO S. A. LEITE, UNB. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Health
Toxicology and Mutagenesis medicine.medical_treatment lcsh:Medicine 010501 environmental sciences Biology Peptídeos antimicrobianos 01 natural sciences Article Microbiology 03 medical and health sciences Mice antimicrobial peptides 0302 clinical medicine Immune system In vivo parasitic diseases medicine cytokine Leishmaniose Animals oxidative stress Secretion 030212 general & internal medicine Stress oxidativo Amastigote Cytokine Leishmaniasis leishmaniasis 0105 earth and related environmental sciences Leishmania Mice Inbred BALB C Macrophages lcsh:R Public Health Environmental and Occupational Health Citocinas medicine.disease biology.organism_classification In vitro Oxidative stress Macrophages Peritoneal Antimicrobial peptides Female |
| Zdroj: | International Journal of Environmental Research and Public Health, Vol 17, Iss 4856, p 4856 (2020) International Journal of Environmental Research and Public Health Volume 17 Issue 13 Repositório Institucional da UnB Universidade de Brasília (UnB) instacron:UNB Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| ISSN: | 1661-7827 1660-4601 |
| Popis: | Leishmania protozoans are the causal agents of neglected diseases that represent an important public health issue worldwide. The growing occurrence of drug-resistant strains of Leishmania and severe side effects of available treatments represent an important challenge for the leishmaniases treatment. We have previously reported the leishmanicidal activity of phylloseptin-1 (PSN-1), a peptide found in the skin secretion of Phyllomedusa azurea (=Pithecopus azureus), against Leishmania amazonensis promastigotes. However, its impact on the amastigote form of L. amazonensis and its impact on infected macrophages are unknown. In this work, we evaluated the effects of PSN-1 on amastigotes of L. amazonensis inside macrophages infected in vitro. We assessed the production of hydrogen peroxide and nitric oxide, as well as the levels of inflammatory and immunomodulatory markers (TGF-&beta TNF-&alpha and IL-12), in infected and non-infected macrophages treated with PSN-1. Treatment with PSN-1 decreased the number of infected cells and the number of ingested amastigotes per cell when compared with the untreated cells. At 32 µ M (64 µ g/mL), PSN-1 reduced hydrogen peroxide levels in both infected and uninfected macrophages, whereas it had little effect on NO production or TGF-&beta release. The effect of PSN-1 on IL-12 and TNF-&alpha secretion depended on its concentration, but, in general, their levels tended to increase as PSN-1 concentration increased. Further in vitro and in vivo studies are needed to clarify the mechanisms of action of PSN-1 and its interaction with the immune system aiming to develop pharmacological applications. |
| Databáze: | OpenAIRE |
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