A novel lipopeptide, an inhibitor of bacterial adhesion, from the thermophilic and halotolerant subsurface Bacillus licheniformis strain 603
| Autor: | Stanislav E. Esipov, Yuri A. Nikolaev, Sergey M Lukin, Tatiana A Rodionova, Stanislav G. Batrakov, Nina V. Shekhovtsova, Nikolai S Panikov, Vladimir I Sheichenko, N. B. Polyakov |
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| Rok vydání: | 2003 |
| Předmět: |
Metabolite
Chemical structure Lipoproteins Bacillus Corynebacterium Peptides Cyclic Bacterial Adhesion chemistry.chemical_compound Bacillus licheniformis Amino Acid Sequence Molecular Biology Soil Microbiology chemistry.chemical_classification Growth medium Chromatography biology Strain (chemistry) Acinetobacter Molecular Structure Spectrum Analysis Fatty acid Lipopeptide Cell Biology biology.organism_classification Amino acid chemistry Biochemistry |
| Zdroj: | Biochimica et biophysica acta. 1634(3) |
| ISSN: | 0006-3002 |
| Popis: | A new Bacillus licheniformis strain, 603, isolated from a mixture of drilling fluid and subsurface thermal water, has been found to produce a cyclic lipopeptide which is released into cultural medium as well as present in cells as the major lipid constituent (57% of the total cell lipids extractable with 2:1 chloroform–methanol). The quantitative ratio of the extracellular and intracellular lipopeptide has been estimated as 23:10. The metabolite represents a heptapeptide, l -Asp→ l -Leu→ l -Leu→ l -Val→ l -Val→ l -Glu→ l -Leu, N-acylated to the N-terminal amino acid, l -Asp, by a 3-hydroxy fatty acid (from 13:0 to 17:0 with n-, iso-, and anteiso-chains), the 3-OH group of which is esterified by the C-terminal amino acid, l -Leu. The chemical structure of the lipopeptide has been established by means of infrared (IR), 1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy, electrospray ionisation (ESI) mass spectrometry (MS), including secondary ion mass spectrometry, along with chemical and enzymatic degradation. Although a diversity of similar metabolites synthesised by various B. licheniformis strains are presently known, such a structure has not been reported thus far. Added to the growth medium of strain 603 at the concentration of 1.6 μg/ml, the lipopeptide prevents adhesion of cells to a glass surface. Also, it exhibits a considerable growth-inhibiting activity against Corynebacterium variabilis and a much lower activity against Acinetobacter sp. |
| Databáze: | OpenAIRE |
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