Raman spectroscopy based molecular signatures of methamphetamine and HIV induced mitochondrial dysfunction

Autor:	Khoo Ting Chean, Ravikumar Aalinkeel, Serfraz Abbasi, Anna V. Sharikova, Stanley A. Schwartz, Alexander Khmaladze, Supriya D. Mahajan
Rok vydání:	2022
Předmět:	Biophysics Humans HIV Infections tat Gene Products Human Immunodeficiency Virus Cell Biology Spectrum Analysis Raman Molecular Biology Biochemistry Methamphetamine Mitochondria
Zdroj:	Biochemical and Biophysical Research Communications. 621:116-121
ISSN:	0006-291X
DOI:	10.1016/j.bbrc.2022.06.098
Popis:	METH and HIV Tat treatment results in increased oxidative stress which affects cellular metabolism and causes DNA damage in the treated microglia. Both, METH ± HIV Tat impair mitochondrial respiration, leading to dysfunction in bioenergetics and increased ROS in microglial cells. Our data indicate that mitochondrial dysfunction may be key to the METH and/or HIV Tat-induced neuropathology. METH and/or HIV Tat induced changes in the protein, lipid and nucleotide concentration in microglial cells were measured by Raman Spectroscopy, and we speculate that these fundamental molecular-cellular changes in microglial cells contribute to the neuropathology that is associated with METH abuse in HIV patients.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f76ffbe5a33d5570d236b021fc7aa4a5 https://doi.org/10.1016/j.bbrc.2022.06.098 Zobrazit plný text záznamu Full Text from ScienceDirect