Purification, characterization, and mode of action of a novel bacteriocin BM173 from Lactobacillus crustorum MN047 and its effect on biofilm formation of Escherichia coli and Staphylococcus aureus
| Autor: | Bianfang Liu, Yuanyuan Shan, Yanglei Yi, Zhu Qiao, Qiaqia Zhou, Yuan Zhou, Xin Lü, Xin Wang, Jiaxin Chen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Staphylococcus aureus
Cell Membrane Permeability Lysis Membrane permeability Microbial Sensitivity Tests medicine.disease_cause 03 medical and health sciences Minimum inhibitory concentration Bacteriocins Bacteriocin Escherichia coli Genetics medicine Food science 030304 developmental biology 0303 health sciences biology Chemistry 0402 animal and dairy science Biofilm 04 agricultural and veterinary sciences biology.organism_classification 040201 dairy & animal science Anti-Bacterial Agents Lactobacillus Biofilms Food Preservatives Animal Science and Zoology Antibacterial activity Bacteria Food Science |
| Zdroj: | Journal of Dairy Science. 104:1474-1483 |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.2020-18959 |
| Popis: | There is an increasing demand for dairy products, but the presence of food-spoilage bacteria seriously affects the development of the dairy industry. Bacteriocins are considered to be a potential antibacterial or antibiofilm agent that can be applied as a preservative. In this study, bacteriocin BM173 was successfully expressed in the Escherichia coli expression system and purified by a 2-step method. Furthermore, it exhibited a broad-spectrum antibacterial activity, high thermal stability (121°C, 20 min), and broad pH stability (pH 3-11). Moreover, the minimum inhibitory concentration values of BM173 against E. coli ATCC 25922 and Staphylococcus aureus ATCC 25923 were 14.8 μg/mL and 29.6 μg/mL, respectively. Growth and time-kill curves showed that BM173 exhibited antibacterial and bactericidal activity. The results of scanning electron microscopy and transmission electron microscopy demonstrated that BM173 increased membrane permeability, facilitated pore formation, and even promoted cell lysis. The disruption of cell membrane integrity was further verified by propidium iodide uptake and lactic dehydrogenase release. In addition, BM173 exhibited high efficiency in inhibiting biofilm formation. Therefore, BM173 has promising potential as a preservative used in the dairy industry. |
| Databáze: | OpenAIRE |
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