The herbicide atrazine affects sperm quality and the expression of antioxidant and spermatogenesis genes in zebrafish testes

Autor: Ednei Gilberto Primel, Felix Esteban Airahuacho Bautista, Antonio Sergio Varela Junior, Carine Dahl Corcini, Juliano Zanette, Izani Bonel Acosta, Sergiane Souza Caldas
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Saccharomyces cerevisiae Proteins
Physiology
DNA repair
Health
Toxicology and Mutagenesis
Cystic Fibrosis Transmembrane Conductance Regulator
Cell Cycle Proteins
Mitochondrion
Toxicology
Biochemistry
Superoxide dismutase
Random Allocation
03 medical and health sciences
0302 clinical medicine
3-Oxo-5-alpha-Steroid 4-Dehydrogenase
Testis
Animals
Spermatogenesis
Zebrafish
Gene
Sperm motility
Glutathione Peroxidase
Xeroderma Pigmentosum
030219 obstetrics & reproductive medicine
biology
Herbicides
Superoxide Dismutase
Osmolar Concentration
Gene Expression Regulation
Developmental
Membrane Proteins
Cell Biology
General Medicine
Zebrafish Proteins
Phospholipid Hydroperoxide Glutathione Peroxidase
biology.organism_classification
Sperm
Mitochondria
Cell biology
030104 developmental biology
Mitochondrial Membranes
Sperm Motility
biology.protein
Atrazine
Water Pollutants
Chemical
Zdroj: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. :17-22
ISSN: 1532-0456
DOI: 10.1016/j.cbpc.2018.02.003
Popis: The herbicide atrazine (ATZ) is used worldwide in the control of annual grasses and broad-leaved weeds. The present study evaluated sperm quality parameters in zebrafish Danio rerio after 11-day exposure to nominal ATZ concentrations of 2, 10, and 100 μg L-1. All ATZ concentrations caused a decrease in motility, mitochondrial functionality, and membrane integrity, as measured using conventional microscopy or fluorescence microscopy with specific probes. The DNA integrity of sperm was not affected. The levels of expression of genes related to spermatogenesis, antioxidant defenses, and DNA repair were also investigated using RT-qPCR. The ATZ caused transcriptional repression of the spermatogenesis-related genes SRD5A2 and CFTR, the antioxidant defense genes SOD2 and GPX4B, and the DNA repair gene XPC. This is the first study to show that environmentally relevant concentrations of ATZ significantly affect the sperm quality in fish, possibly resulting in reduced fertility rates. In addition, we showed that the repression of genes related to spermatogenesis and cellular defense could be part of the mechanisms involved in the ATZ toxicity in the testes of male fish.
Databáze: OpenAIRE
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