A monoclonal antibody to murine CD45R distinguishes CD4 T cell populations that produce different cytokines
| Autor: | Theresa Pasqualini, Donal B. Murphy, Mohammad Luqman, Jeff West, Laurence Greenbaum, Simon R. Carding, Kim Bottomly |
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| Rok vydání: | 1989 |
| Předmět: |
Interleukin 2
CD4-Positive T-Lymphocytes T cell Immunology Mice Inbred Strains Streptamer Biology Lymphocyte Activation Interleukin 21 Biological Factors Mice Antigen medicine Immunology and Allergy Cytotoxic T cell Animals Interleukin 4 Interleukins Antibodies Monoclonal T lymphocyte Flow Cytometry Molecular biology Antigens Differentiation Precipitin Tests Molecular Weight medicine.anatomical_structure Cytokines Interleukin-2 Leukocyte Common Antigens Interleukin-4 Spleen medicine.drug |
| Zdroj: | European journal of immunology. 19(4) |
| ISSN: | 0014-2980 |
| Popis: | CD4 T cell clones have been shown to be functionally heterogeneous in the mouse. However, it is not known if normal CD4 T cells are also functionally heterogeneous, or whether functional specialization is a result of cloning and long-term culture. To approach this question, a monoclonal antibody reacting with a subset of CD4 T cells has been prepared by immunization of rats with different cloned T cell lines all sharing the same functional activity. This monoclonal antibody reacts with a subset of CD45 (T200) molecules by binding to a determinant requiring the expression of the second variable exon of the CD45 molecule. Some CD4 T cells bear high levels of this marker, while others react only weakly. This antibody was used to separate CD4 T cells into two subpopulations. The brightly staining population was found to produce interleukin (IL) 2 and not IL 4, while the weakly staining population produced IL 4 and not IL 2. These data demonstrate that CD4 T cells in normal mice are already functionally committed, and that they differentially express forms of CD45 that contain the second variable exon. |
| Databáze: | OpenAIRE |
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