Beneficial effects of fermented black ginseng and its ginsenoside 20(S)-Rg3 against cisplatin-induced nephrotoxicity in LLC-PK1 cells
| Autor: | Su-Nam Kim, I. K. Han, Myoung-Sook Shin, Noriko Yamabe, Jun Min An, Hyuk-Jai Jang, Gwi Seo Hwang, Myoung-Sik Han, Suk-Jung Choi, Ki Sung Kang, Hye Hyun Yoo, Da Hae Lee |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
cisplatin Pharmacology Biochemistry Genetics and Molecular Biology (miscellaneous) Nephrotoxicity 03 medical and health sciences Ginseng chemistry.chemical_compound 0302 clinical medicine lcsh:Botany Medicine Viability assay Cisplatin Kidney ginsenoside 20(S)-Rg3 urogenital system business.industry Kinase nephrotoxicity Panax ginseng lcsh:QK1-989 030104 developmental biology medicine.anatomical_structure Complementary and alternative medicine chemistry Ginsenoside Apoptosis mitogen-activated protein kinases 030220 oncology & carcinogenesis business Research Article Biotechnology medicine.drug |
| Zdroj: | Journal of Ginseng Research, Vol 40, Iss 2, Pp 135-140 (2016) Journal of Ginseng Research |
| ISSN: | 1226-8453 |
| DOI: | 10.1016/j.jgr.2015.06.006 |
| Popis: | Background Nephrotoxicity is a common side effect of medications. Panax ginseng is one of the best-known herbal medicines, and its individual constituents enhance renal function. Identification of its efficacy and mechanisms of action against drug-induced nephrotoxicity, as well as the specific constituents mediating this effect, have recently emerged as an interesting research area focusing on the kidney protective efficacy of P. ginseng . Methods The present study investigated the kidney protective effect of fermented black ginseng (FBG) and its active component ginsenoside 20(S)-Rg3 against cisplatin (chemotherapy drug)-induced damage in pig kidney (LLC-PK1) cells. It focused on assessing the role of mitogen-activated protein kinases as important mechanistic elements in kidney protection. Results The reduced cell viability induced by cisplatin was significantly recovered with FBG extract and ginsenoside 20(S)-Rg3 dose-dependently. The cisplatin-induced elevated protein levels of phosphorylated c-Jun N-terminal kinase (JNK), p53, and cleaved caspase-3 were decreased after cotreatment with FBG extract or ginsenoside 20(S)-Rg3. The elevated percentage of apoptotic LLC-PK1 cells induced by cisplatin treatment was significantly abrogated by cotreatment with FBG and the ginsenoside 20(S)-Rg3. Conclusion FBG and its major ginsenoside 20(S)-Rg3, ameliorated cisplatin-induced nephrotoxicity in LLC-PK1 cells by blocking the JNK–p53–caspase-3 signaling cascade. |
| Databáze: | OpenAIRE |
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