Efficacy of nanoemulsion with Pterodon emarginatus Vogel oleoresin for topical treatment of cutaneous leishmaniasis

Autor: Marta Gontijo Aguiar, Monique Y.M. Kawakami, Raquel Silva Araújo, Leandro G. Oliveira, Caio P. Fernandes, Anna E.M.F.M. Oliveira, Ana Paula Fernandes, Celso Martins Queiroz-Junior, Lucas Antônio Miranda Ferreira, André Luís Branco de Barros, Lisset Ortiz Zamora, Edemilson Cardoso da Conceição, Tiago Queiroga Nery Ricotta
Rok vydání: 2021
Předmět:
0301 basic medicine
Administration
Topical
Meglumine antimoniate
Leishmania mexicana
Drug resistance
Pharmacology
Parasite Load
chemistry.chemical_compound
0302 clinical medicine
Pterodon emarginatus
Skin
Mice
Inbred BALB C
Meglumine Antimoniate
biology
Fabaceae
General Medicine
Trypanocidal Agents
Neglected diseases
030220 oncology & carcinogenesis
Drug delivery
Cytokines
Drug Therapy
Combination
Emulsions
Female
medicine.symptom
medicine.drug
Nanoemulsions
Drug Compounding
Leishmaniasis
Cutaneous
RM1-950
Host-Parasite Interactions
Lesion
03 medical and health sciences
Cutaneous leishmaniasis
Topical treatment
Brazilian species
medicine
Animals
Oleoresin
Mesocricetus
Plant Extracts
biology.organism_classification
medicine.disease
Terpenoid
Disease Models
Animal
030104 developmental biology
chemistry
Nanoparticles
Therapeutics. Pharmacology
Zdroj: Biomedicine & Pharmacotherapy, Vol 134, Iss, Pp 111109-(2021)
ISSN: 0753-3322
DOI: 10.1016/j.biopha.2020.111109
Popis: Cutaneous leishmaniasis (CL) is a neglected tropical skin disease caused by the protozoan genus Leishmania. The treatment is restricted to a handful number of drugs that exhibit toxic effects, limited efficacy, and drug resistance. Additionally, developing an effective topical treatment is still an enormous unmet medical challenge. Natural oils, e.g. the oleoresin from P. emarginatus fruits (SO), contain various bioactive molecules, especially terpenoid compounds such as diterpenes and sesquiterpenes. However, its use in topical formulations can be impaired due to the natural barrier of the skin for low water solubility compounds. Nanoemulsions (NE) are drug delivery systems able to increase penetration of lipophilic compounds throughout the skin, improving their topical effect. In this context, we propose the use of SO-containing NE (SO-NE) for CL treatment. The SO-NE was produced by a low energy method and presented suitable physicochemical characteristic: average diameter and polydispersity index lower than 180 nm and 0.2, respectively. Leishmania (Leishmania) amazonensis-infected BALB/c mice were given topical doses of SO or SO-NE. The topical use of a combination of SO-NE and intraperitoneal meglumine antimoniate reduced lesion size by 41 % and tissue regeneration was proven by histopathological analyses. In addition, a reduction in the parasitic load and decreased in the level of IFN-γ in the lesion may be associated, as well as a lower level of the cytokine IL-10 may be associated with a less intense inflammatory process. The present study suggests that SO-NE in combination meglumine antimoniate represents a promising alternative for the topical treatment of CL caused by L. (L.) amazonensis.
Databáze: OpenAIRE
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