Inhibition of Inflammatory Cytokine Production in Human Corneal Cells by Dexamethasone, but Not Cyclosporin
| Autor: | Robert B. Nussenblatt, John J. Hooks, Janine A. Smith, Sankanaranayana P Mahesh, Ali R. Djalilian, Chandrasekharam N. Nagineni |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Chemokine
Corneal Stroma medicine.medical_treatment Interleukin-1beta Anti-Inflammatory Agents Cell Culture Techniques Gene Expression Stimulation Inflammation Dexamethasone Cyclosporin a polycyclic compounds medicine Humans RNA Messenger Chemokine CCL7 Chemokine CCL5 Chemokine CCL2 biology Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Interleukin-8 Epithelium Corneal Granulocyte-Macrophage Colony-Stimulating Factor Fibroblasts In vitro Monocyte Chemoattractant Proteins Ophthalmology Cytokine Cell culture Immunology Cyclosporine biology.protein Cytokines medicine.symptom Immunosuppressive Agents medicine.drug |
| Zdroj: | Cornea. 25:709-714 |
| ISSN: | 0277-3740 |
| DOI: | 10.1097/01.ico.0000208815.02120.90 |
| Popis: | To evaluate the modulatory effects of anti-inflammatory agents, dexamethasone (Dex) and cyclosporin A (CsA), on the production of cytokines and chemokines by human corneal cells in vitro following stimulation by the pro-inflammatory cytokine after interleukin 1beta (IL-1beta).A human corneal epithelial (HCE) cell line and human corneal fibroblasts (HCFs) were stimulated in culture with IL-1beta and treated with Dex or CsA. The gene expression for selected cytokines and chemokines was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). The secretion of cytokines and chemokines was measured by enzyme-linked immunosorbent assay.IL-1beta enhanced the mRNA and/or protein levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-8, and monocyte chemotactic protein (MCP)-1 in HCE and IL-6, IL-8, MCP-3, and regulated on T-cell activation expressed secreted (RANTES) in HCFs. Treatment with CsA did not inhibit cytokine production in either HCE or HCFs. In contrast, Dex treatment inhibited the IL-1beta-induced production of GM-CSF, IL-6, IL-8, MCP-3, and RANTES, but not MCP-1.These results show that Dex, but not CsA, has direct immunosuppressive effects on the resident corneal cells, HCE and HCFs. This suggests that the clinically observed immunosuppressive effects of topical CsA are mediated primarily through the immune cells. |
| Databáze: | OpenAIRE |
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