Xenopus laevis FGF16 activates the expression of genes coding for the transcription factors Sp5 and Sp5l
| Autor: | Hannah Lord, Harry V. Isaacs, Michael Elsy, Mary Elizabeth Pownall, Abigail Rowbotham |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Embryology
Mesoderm Embryo Nonmammalian animal structures MAP Kinase Signaling System Xenopus Xenopus Proteins Biology Fibroblast growth factor Xenopus laevis 03 medical and health sciences medicine Animals In Situ Hybridization Phylogeny 030304 developmental biology Regulation of gene expression 0303 health sciences Embryogenesis Gene Expression Regulation Developmental Gastrula biology.organism_classification Cell biology Fibroblast Growth Factors Gastrulation Branchial Region medicine.anatomical_structure embryonic structures Mesoderm formation Otic vesicle Transcription Factors Developmental Biology |
| ISSN: | 1696-3547 |
| Popis: | Fibroblast growth factors (FGFs) comprise a family of signalling molecules with essential roles in early embryonic development across animal species. The role of FGFs in mesoderm formation and patterning in Xenopus has been particularly well studied. However, little is known about FGF16 in Xenopus. Using in situ hybridisation, we uncover the expression pattern of FGF16 during early Xenopus laevis development, which has not been previously described. We show that the zygotic expression of FGF16 is activated in the mesoderm of the early gastrula as a ring around the blastopore, with its first accumulation at the dorsal side of the embryo. Later, FGF16 expression is found in the otic vesicle, the branchial arches and the anterior pituitary, as well as in the chordal neural hinge region of the tailbud. In addition, we show that FGF16 can activate the MAPK pathway and expression of sp5 and sp5l. Like FGF16, sp5 is expressed in the otic vesicle and the branchial arches, with all three of these genes being expressed in the tailbud. These data provide evidence that FGF16 is present in the early mesoderm and can activate the expression of developmentally important transcription factors. |
| Databáze: | OpenAIRE |
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