Detection of Dobrava-Belgrade hantavirus using recombinant-nucleocapsid-based enzyme-linked immunosorbent assay and SYBR Green-based real-time reverse transcriptase-polymerase chain reaction
| Autor: | Miklós Oldal, Viktória Németh, Mónika Madai, Péter Kisfali, Ferenc Jakab, Győző Horváth, Bálint Bérczi, Alexandra Maráczi, Krisztián Bányai |
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| Rok vydání: | 2011 |
| Předmět: |
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Orthohantavirus Croatia viruses Enzyme-Linked Immunosorbent Assay Biology law.invention Serology Rodent Diseases law Virology Animals Polymerase chain reaction Phylogeny Fluorescent Dyes chemistry.chemical_classification Hungary Reverse Transcriptase Polymerase Chain Reaction Dobrava-Belgrade hantavirus virus diseases General Medicine biology.organism_classification Reverse transcriptase Enzyme chemistry Apodemus Recombinant DNA Female Murinae Hantavirus Infection |
| Zdroj: | Archives of virology. 156(9) |
| ISSN: | 1432-8798 |
| Popis: | Dobrava (DOBV) hantaviruses belong to the genus Hantavirus, family Bunyaviridae, and are carried by yellow-necked and striped field mice. The goal of this study was to detect DOBV using serological and genetic methods in Apodemus rodents in Hungary and in northern Croatia. During the study period, a total of 125 Apodemus sp. (67 A. agrarius, 58 A. flavicollis) were tested for the presence of hantaviruses, and 21 rodents (17%) were positive by rRT-PCR and/or ELISA. We conclude that the prevalence of DOBV is much higher than previously anticipated. The simultaneous use of molecular and serological techniques provides a highly reliable way to detect hantavirus infections. |
| Databáze: | OpenAIRE |
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