Efficacy of Combined Vancomycin and Fosfomycin against Methicillin-Resistant Staphylococcus aureus in Biofilms In Vivo
| Autor: | Li Wang, Qian Chen, Jian Shi, Fu-Bing Li, Xun Tang, Han-Bo Zhang, Hua Liu, Chong-Tao Zhu, Yong-Qing Xu, Ning-Fang Mao, Xin-Ming Xu, Tao Jin, Lin-Hui Sun |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Methicillin-Resistant Staphylococcus aureus medicine.drug_class Antibiotics lcsh:Medicine Microbial Sensitivity Tests Fosfomycin medicine.disease_cause Staphylococcal infections Microbiology Rats Sprague-Dawley Leukocyte Count Cryobiology Vancomycin In vivo Staphylococcus epidermidis medicine Animals lcsh:Science Multidisciplinary biology business.industry lcsh:R Biology and Life Sciences Drug Synergism Cell Biology Staphylococcal Infections biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification Methicillin-resistant Staphylococcus aureus Anti-Bacterial Agents Rats C-Reactive Protein Staphylococcus aureus Biofilms lcsh:Q business Research Article medicine.drug |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 12, p e113133 (2014) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0113133 |
| Popis: | Infection by methicillin-resistant Staphylococcus aureus (MRSA) is a life-threatening condition, and formation of biofilms can lead to treatment failure in a clinical setting. The aim of this study was to demonstrate the in vivo bactericidal effects of a combination of vancomycin (VAN) and fosfomycin (FOS) against MRSA in a rat carboxymethyl cellulose-pouch biofilm model. The results of the time-kill assay showed that the combination therapy was capable of killing at low minimal inhibitory concentrations (MIC) (½ × MIC VAN +1 × MIC FOS and 1 × MIC VAN + 1 × MIC FOS). In the in vivo study, a synergistically bactericidal effect was observed when using the combination therapy on MRSA embedded in the mature biofilm model. In comparison with the untreated control group and the groups receiving either VAN or FOS alone, the rats treated with combination therapy had lower MRSA colony counts in exudates from the pouch, lower white blood cell and neutrophil counts, and C-reactive protein (CRP) in peripheral blood. Furthermore, histological analysis of the pouch wall indicated combination therapy resulted in disappearance of biofilm-like structures, marked decrease in necrosis, and formation of granular tissue. In conclusion, the combination of VAN with FOS had a synergistic bactericidal effect on chronic MRSA infection embedded in biofilm, providing an alternative approach to treating this condition. |
| Databáze: | OpenAIRE |
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