Engineering of Acid-Resistant d-Allulose 3-Epimerase for Functional Juice Production

Autor:	Lei Li, Qianqian Zhang, Tong Wang, Hongbin Qi, Meijing Wei, Fuping Lu, Lijun Guan, Shuhong Mao, Hui-Min Qin
Rok vydání:	2022
Předmět:	Sweetening Agents Racemases and Epimerases Fructose General Chemistry General Agricultural and Biological Sciences
Zdroj:	Journal of Agricultural and Food Chemistry. 70:16298-16306
ISSN:	1520-5118 0021-8561
DOI:	10.1021/acs.jafc.2c07153
Popis:	d-Allulose, a rare sugar and functional sweetener, can be biosynthesized by d-allulose 3-isomerase (DAE). However, most of the reported DAEs exhibit poor resistance under acidic conditions, which severely limited their application. Here, surface charge engineering and random mutagenesis were used to construct a mutant library of CcDAE from
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6d0f582c6587e62cd71320c3dc35ce6 https://doi.org/10.1021/acs.jafc.2c07153 Zobrazit plný text záznamu